biostudies-literatureOtiniano NMVicerrectorado de Investigación, Universidad César Vallejo27223https://www.ebi.ac.uk/biostudies/studies/S-EPMC11549403biostudies-literatureUnknown14(1)The self-healing of concrete through the use of bacteria is an ecological, sustainable, and environmentally friendly method that can replace time-consuming and costly repairs. The aim of this research was to isolate and select spore-forming bacilli with potential for concrete self-healing. Soil samples from the limestone mines of Simbal, Trujillo, La Libertad, Peru were used. These samples were processed to primarily isolate spore-forming bacteria. The self-healing potential of these bacteria was evaluated through their ability to produce the urease enzyme, grow at an alkaline pH, and produce calcium carbonate crystals in a culture medium with urea and CaCl₂ at pH 8.0. The produced crystals were visualized with scanning electron microscopy and their calcium content was confirmedScientific reportsIsolation and selection of spore-forming bacilli with potential for self-healing of concrete.PMC11549403RVI N°299-2022-VI-UCVCabanillas-Chirinos LFarfan-Cordova MOtiniano NMCabeza JGfalseIsolation and selection of spore-forming bacilli with potential for self-healing of concrete.The self-healing of concrete through the use of bacteria is an ecological, sustainable, and environmentally friendly method that can replace time-consuming and costly repairs. The aim of this research was to isolate and select spore-forming bacilli with potential for concrete self-healing. Soil samples from the limestone mines of Simbal, Trujillo, La Libertad, Peru were used. These samples were processed to primarily isolate spore-forming bacteria. The self-healing potential of these bacteria was evaluated through their ability to produce the urease enzyme, grow at an alkaline pH, and produce calcium carbonate crystals in a culture medium with urea and CaCl₂ at pH 8.0. The produced crystals were visualized with scanning electron microscopy and their calcium content was confirmed2024-01-01T00:00:00Z2024 Nov2026-07-15T10:58:29.565Z2025-04-19T17:27:44.674ZS-EPMC115494033951624010.1038/s41598-024-77241-9