biostudies-literatureUnknown14(1)Huang QAs a traditional Chinese medicine formula used in clinical practice for an extended period, Suxin-Hugan-Fang (SXHGF) exhibits excellent anti-inflammatory properties. However, the efficacy of SXHGF in treating ulcerative colitis (UC) and its mechanism of action are still unclear. In this study, the therapeutic effects of SXHGF on UC were evaluated using network pharmacology and experimental validation, while also investigating its mechanism of action. By administering DSS to C57BL/6 mice to construct a mouse model of ulcerative colitis, the therapeutic effect of SXHGF on ulcerative colitis was evaluated based on weight loss percentage, disease activity index, colon length changes, and pathological conditions as indicators. The main chemical components of SXHGF were determined by LC-MS-QTOF Scientific reports27196https://www.ebi.ac.uk/biostudies/studies/S-EPMC11549446biostudies-literatureExploring the mechanism of Suxin Hugan Fang in treating ulcerative colitis based on network pharmacology.PMC11549446Luo QPeng WHu XZhao WDai WZeng HHuang QWong HLXfalseExploring the mechanism of Suxin Hugan Fang in treating ulcerative colitis based on network pharmacology.As a traditional Chinese medicine formula used in clinical practice for an extended period, Suxin-Hugan-Fang (SXHGF) exhibits excellent anti-inflammatory properties. However, the efficacy of SXHGF in treating ulcerative colitis (UC) and its mechanism of action are still unclear. In this study, the therapeutic effects of SXHGF on UC were evaluated using network pharmacology and experimental validation, while also investigating its mechanism of action. By administering DSS to C57BL/6 mice to construct a mouse model of ulcerative colitis, the therapeutic effect of SXHGF on ulcerative colitis was evaluated based on weight loss percentage, disease activity index, colon length changes, and pathological conditions as indicators. The main chemical components of SXHGF were determined by LC-MS-QTOF 2024-01-01T00:00:00Z2024 Nov2026-07-16T18:49:51.931Z2025-04-04T18:56:06.437ZS-EPMC115494463951663310.1038/s41598-024-78833-1