biostudies-literatureD'Onofrio FMinistero della Salutee39832https://www.ebi.ac.uk/biostudies/studies/S-EPMC11550065biostudies-literatureUnknown10(21)<i>Listeria monocytogenes</i> is a significant concern in the food industry due to its association with outbreaks of listeriosis, particularly affecting vulnerable populations. High-throughput technologies such as RNA sequencing (RNA-seq) and proteomics offer valuable insights into the molecular responses of <i>L. monocytogenes</i> to stress environments. In this study, a combined transcriptomic and immunoproteomic approach was applied to explore the stress response mechanisms of the <i>L. monocytogenes</i> strain ST7, which was responsible for an outbreak in central Italy. The bacterium was exposed to both optimal conditions and a stress environment representative of pork product matrices (pH 5.5; 7 % NaCl) and thermal abuse prior to consumption (12 °C).Transcriptomic analysis revealed vaHeliyonIntegrative analysis of transcriptomic and immunoproteomic data reveals stress response mechanisms in <i>Listeria monocytogenes</i>.PMC11550065IZS AM 03/17 RCMSRCTE0719Tittarelli MLuciani MMarcacci MPomilio FButler FDi Lollo VTorresi MIannetti LKrasteva IDi Pancrazio CD'Onofrio FAncora MSchirone MD'Alterio NMaggetti MCamma CPerletta FDi Domenico MSacchini FfalseIntegrative analysis of transcriptomic and immunoproteomic data reveals stress response mechanisms in <i>Listeria monocytogenes</i>.<i>Listeria monocytogenes</i> is a significant concern in the food industry due to its association with outbreaks of listeriosis, particularly affecting vulnerable populations. High-throughput technologies such as RNA sequencing (RNA-seq) and proteomics offer valuable insights into the molecular responses of <i>L. monocytogenes</i> to stress environments. In this study, a combined transcriptomic and immunoproteomic approach was applied to explore the stress response mechanisms of the <i>L. monocytogenes</i> strain ST7, which was responsible for an outbreak in central Italy. The bacterium was exposed to both optimal conditions and a stress environment representative of pork product matrices (pH 5.5; 7 % NaCl) and thermal abuse prior to consumption (12 °C).Transcriptomic analysis revealed va2024-01-01T00:00:00Z2024 Nov2026-06-01T17:33:30.809Z2025-04-19T17:28:46.075ZS-EPMC115500653952475410.1016/j.heliyon.2024.e39832