biostudies-literatureLiu QNational Natural Science Foundation of ChinaCzech Science Foundationycae127https://www.ebi.ac.uk/biostudies/studies/S-EPMC11550332biostudies-literatureUnknown4(1)Endosymbiotic bacteria significantly impact the fitness of their arthropod hosts. <i>Dermanyssus gallinae</i>, the poultry red mite, is a blood-feeding ectoparasite that exclusively feeds on avian blood. While there is a relatively comprehensive understanding of its microbial community structures across developmental stages based on 16S rRNA sequencing， the functional integration of these microbes within the host's physiology remains elusive. This study aims to elucidate the role of symbiotic bacteria in <i>D. gallinae</i> biology. 16S rRNA amplicon sequencing and fluorescence <i>in situ</i> hybridization revealed a prominent midgut-confinement bacterial microbiota with considerable diversity, out of which <i>Kocuria</i> and <i>Bartonella</i> A acted as the predominant bacterial genera inhISME communicationsSignificant role of symbiotic bacteria in the blood digestion and reproduction of <i>Dermanyssus gallinae</i> mites.PMC1155033222-18424M32273027Perner JWang PFrantova HSun TLiu QHartmann DPan BSun WWang LfalseSignificant role of symbiotic bacteria in the blood digestion and reproduction of <i>Dermanyssus gallinae</i> mites.Endosymbiotic bacteria significantly impact the fitness of their arthropod hosts. <i>Dermanyssus gallinae</i>, the poultry red mite, is a blood-feeding ectoparasite that exclusively feeds on avian blood. While there is a relatively comprehensive understanding of its microbial community structures across developmental stages based on 16S rRNA sequencing， the functional integration of these microbes within the host's physiology remains elusive. This study aims to elucidate the role of symbiotic bacteria in <i>D. gallinae</i> biology. 16S rRNA amplicon sequencing and fluorescence <i>in situ</i> hybridization revealed a prominent midgut-confinement bacterial microbiota with considerable diversity, out of which <i>Kocuria</i> and <i>Bartonella</i> A acted as the predominant bacterial genera inh2024-01-01T00:00:00Z2024 Jan2026-07-15T08:12:52.212Z2025-04-06T22:45:59.853ZS-EPMC115503323952613210.1093/ismeco/ycae127