{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Girao H"],"funding":["\"la Caixa\" Foundation (Caixa Foundation)","Ministry of Education and Science | Fundação para a Ciência e a Tecnologia (Portuguese Science and Technology Foundation)","European Research Council","U.S. Department of Health & Human Services | National Institutes of Health (NIH)","NIGMS NIH HHS"],"pagination":["9720"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11550433"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Post-translational cycles of α-tubulin detyrosination and tyrosination generate microtubule diversity, the cellular functions of which remain largely unknown. Here we show that α-tubulin detyrosination regulates kinetochore-microtubule attachments to ensure normal chromosome oscillations and timely anaphase onset during mitosis. Remarkably, detyrosinated α-tubulin levels near kinetochore microtubule plus-ends depend on the direction of chromosome motion during metaphase. Proteomic analyses unveil that the KNL-1/MIS12/NDC80 (KMN) network that forms the core microtubule-binding site at kinetochores and the microtubule-rescue protein CLASP2 are enriched on tyrosinated and detyrosinated microtubules during mitosis, respectively. α-tubulin detyrosination enhances CLASP2 binding and NDC80 comple"],"journal":["Nature communications"],"pubmed_title":["α-tubulin detyrosination fine-tunes kinetochore-microtubule attachments."],"pmcid":["PMC11550433"],"funding_grant_id":["SFRH/BD/52044/2012","SFRH/BD/141066/2018","PTDC/MED-ONC/3479/2020","R35 GM141747","681443","LCF/PR/HR21/52410025","2021.07945.BD","R35-GM141747"],"pubmed_authors":["Meraldi P","Silva E Sousa R","Macario-Monteiro J","Demidov V","Figueiredo AC","Girao H","Doria E","Osorio H","Jacome A","Maiato H","Grishchuk EL"],"additional_accession":[]},"is_claimable":false,"name":"α-tubulin detyrosination fine-tunes kinetochore-microtubule attachments.","description":"Post-translational cycles of α-tubulin detyrosination and tyrosination generate microtubule diversity, the cellular functions of which remain largely unknown. Here we show that α-tubulin detyrosination regulates kinetochore-microtubule attachments to ensure normal chromosome oscillations and timely anaphase onset during mitosis. Remarkably, detyrosinated α-tubulin levels near kinetochore microtubule plus-ends depend on the direction of chromosome motion during metaphase. Proteomic analyses unveil that the KNL-1/MIS12/NDC80 (KMN) network that forms the core microtubule-binding site at kinetochores and the microtubule-rescue protein CLASP2 are enriched on tyrosinated and detyrosinated microtubules during mitosis, respectively. α-tubulin detyrosination enhances CLASP2 binding and NDC80 comple","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-01T17:41:49.828Z","creation":"2025-04-06T18:28:03.41Z"},"accession":"S-EPMC11550433","cross_references":{"pubmed":["39521805"],"doi":["10.1038/s41467-024-54155-8"]}}