biostudies-literatureMeng LXin Miao Funding of Zunyi Medical UniversityScience & Technology Plan of ZunyiScience and Technology Foundation of Guizhou Province27344https://www.ebi.ac.uk/biostudies/studies/S-EPMC11550443biostudies-literatureUnknown14(1)Natural products, with their extensive chemical diversity, distinctive biological activities, and vast reservoirs, provide a robust foundation for advancing cancer therapeutics. A comprehensive phytochemical investigation of the skins from Bufo bufo gargarizans afforded two new bufadienolide derivatives identified as bufalactamides A and B (1-2), along with six known compounds: argentinogenin (3), desacetylcinobufagin (4), desacetylcinobufaginol (5), cinobufaginol (6), bufalin (7) and gamabufalin (8). The structural elucidation of these compounds was meticulously carried out by analyses of spectroscopic data (1D and 2D NMR, HR-ESIMS), and comparison with the literature data. Plausible biosynthetic pathways for the new compounds were also discussed. Moreover, the cytotoxicity of the compounScientific reportsStructurally diverse bufadienolides from the skins of Bufo bufo gargarizans and their cytotoxicity.PMC11550443QKPTRC[2021]1350-004QKHJC-ZK[2024]YB273ZSKHHZ[2023]168Li CLv HZhang XXiao YDu XMeng LJiang NCao QfalseStructurally diverse bufadienolides from the skins of Bufo bufo gargarizans and their cytotoxicity.Natural products, with their extensive chemical diversity, distinctive biological activities, and vast reservoirs, provide a robust foundation for advancing cancer therapeutics. A comprehensive phytochemical investigation of the skins from Bufo bufo gargarizans afforded two new bufadienolide derivatives identified as bufalactamides A and B (1-2), along with six known compounds: argentinogenin (3), desacetylcinobufagin (4), desacetylcinobufaginol (5), cinobufaginol (6), bufalin (7) and gamabufalin (8). The structural elucidation of these compounds was meticulously carried out by analyses of spectroscopic data (1D and 2D NMR, HR-ESIMS), and comparison with the literature data. Plausible biosynthetic pathways for the new compounds were also discussed. Moreover, the cytotoxicity of the compoun2024-01-01T00:00:00Z2024 Nov2025-04-04T14:50:50.625Z2025-04-04T14:50:50.625ZS-EPMC115504433952192510.1038/s41598-024-79194-5