{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yanagita E"],"funding":["Research Grant of the Princess Takamatsu Cancer Research Fund","Japan Agency for Medical Research and Development"],"pagination":["198-206"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11551815"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["73(5)"],"pubmed_abstract":["The acquisition of high-quality biospecimens and the appropriate handling of these materials are indispensable for successful clinical sequencing. We developed a cancer clinical sequencing system targeting 160 cancer genes: PleSSision-Rapid. Through the PleSSision-Rapid system, we have analyzed DNA quality evaluated by DIN (DNA integrity number) with 1329 formalin-fixed paraffin embedded (FFPE) samples including 477 prospectively collected tissues for genomic test (P) and 852 archival samples after routine pathological diagnosis (A1/A2). As a result, the samples with more than DIN 2.1 was 92.0% (439/477) in prospectively collected sample (P), while it was 85.6% (332/388) and 76.7% (356/464) in two types of archival samples (A1/A2). We performed the PleSSision-Rapid sequence using the sampl"],"journal":["Pathology international"],"pubmed_title":["The DNA integrity number and concentration are useful parameters for successful comprehensive genomic profiling test for cancer using formalin-fixed paraffin embedded tissue."],"pmcid":["PMC11551815"],"funding_grant_id":["21ck0106448S0404"],"pubmed_authors":["Nishihara H","Nakamura K","Kobayashi T","Yanagita E","Yamada H","Aimono E","Hirasawa A"],"additional_accession":[]},"is_claimable":false,"name":"The DNA integrity number and concentration are useful parameters for successful comprehensive genomic profiling test for cancer using formalin-fixed paraffin embedded tissue.","description":"The acquisition of high-quality biospecimens and the appropriate handling of these materials are indispensable for successful clinical sequencing. We developed a cancer clinical sequencing system targeting 160 cancer genes: PleSSision-Rapid. Through the PleSSision-Rapid system, we have analyzed DNA quality evaluated by DIN (DNA integrity number) with 1329 formalin-fixed paraffin embedded (FFPE) samples including 477 prospectively collected tissues for genomic test (P) and 852 archival samples after routine pathological diagnosis (A1/A2). As a result, the samples with more than DIN 2.1 was 92.0% (439/477) in prospectively collected sample (P), while it was 85.6% (332/388) and 76.7% (356/464) in two types of archival samples (A1/A2). We performed the PleSSision-Rapid sequence using the sampl","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-07-11T03:18:14.55Z","creation":"2025-04-04T02:54:02.565Z"},"accession":"S-EPMC11551815","cross_references":{"pubmed":["36971494"],"doi":["10.1111/pin.13318"]}}