biostudies-literatureZhu JYouth Program of Natural Science Foundation of Shandong ProvinceYouth Program of Natural Science Foundation of Qingdao CityNational Key R&D Program of ChinaNational Natural Science Foundation of ChinaState Key Laboratory of Microbial Technology Open Projects FundChina Postdoctoral Science FoundationState Key Laboratory of Microbial TechnologyPostdoctoral Innovation Program of Shandong ProvinceQingdao Postdoctoral Research Project300https://www.ebi.ac.uk/biostudies/studies/S-EPMC11552437biostudies-literatureUnknown23(1)<h4>Background</h4>Acetate is an important chemical feedstock widely applied in the food, chemical and textile industries. It is now mainly produced from petrochemical materials through chemical processes. Conversion of lignocellulose biomass to acetate by biotechnological pathways is both environmentally beneficial and cost-effective. However, acetate production from carbohydrate in lignocellulose hydrolysate via glycolytic pathways involving pyruvate decarboxylation often suffers from the carbon loss and results in low acetate yield.<h4>Results</h4>Escherichia coli BL21 (DE3) was confirmed to have high tolerance to acetate in this work. Thus, it was selected from seven laboratory E. coli strains for acetate production from lignocellulose hydrolysate. The byproduct-producing genes frdA, lMicrobial cell factoriesAcetate production from corn stover hydrolysate using recombinant Escherichia coli BL21 (DE3) with an EP-bifido pathway.PMC11552437M2023-02SKLMTFCP-2023-03No. SDCX-ZG-202400150ZR2022QC092, ZR2023QC237QDBSH202302010112022YFC210600032300045, 323000292023M74208523-2-1-31-zyyd-jchXu PZhang HLu CSun WJiang TKang ZGao CLiu WMeng WLiu YGuo LMa CZhu JTan XTian WfalseAcetate production from corn stover hydrolysate using recombinant Escherichia coli BL21 (DE3) with an EP-bifido pathway.<h4>Background</h4>Acetate is an important chemical feedstock widely applied in the food, chemical and textile industries. It is now mainly produced from petrochemical materials through chemical processes. Conversion of lignocellulose biomass to acetate by biotechnological pathways is both environmentally beneficial and cost-effective. However, acetate production from carbohydrate in lignocellulose hydrolysate via glycolytic pathways involving pyruvate decarboxylation often suffers from the carbon loss and results in low acetate yield.<h4>Results</h4>Escherichia coli BL21 (DE3) was confirmed to have high tolerance to acetate in this work. Thus, it was selected from seven laboratory E. coli strains for acetate production from lignocellulose hydrolysate. The byproduct-producing genes frdA, l2024-01-01T00:00:00Z2024 Nov2025-04-04T00:32:49.439Z2025-04-04T00:32:49.439ZS-EPMC115524373952331610.1186/s12934-024-02575-y