{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shimoda Y"],"funding":["JST-Mirai Program","Japan Society for the Promotion of Science"],"pagination":["27578"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11554776"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Symbiotic nitrogen fixation is a highly coordinated process involving legume plants and nitrogen-fixing bacteria known as rhizobia. In this study, we investigated a novel Fix<sup>-</sup> mutant of the model legume Lotus japonicus that develops root nodules with endosymbiotic rhizobia but fails in nitrogen fixation. Map-based cloning identified the causal gene encoding the filamentation temperature-sensitive H (FtsH) protein, designated as LjFtsH4. The LjFtsH4 gene was expressed in all plant organs without increased levels during nodulation. Subcellular localization revealed that LjFtsH4, fused with a fluorescent protein, localized in mitochondria. The Ljftsh4 mutant nodules showed signs of premature senescence, including symbiosome membrane collapse and bacteroid disintegration. Additional"],"journal":["Scientific reports"],"pubmed_title":["A mitochondrial metalloprotease FtsH4 is required for symbiotic nitrogen fixation in Lotus japonicus nodules."],"pmcid":["PMC11554776"],"funding_grant_id":["21K05335","17570044","JPMJMI20E4"],"pubmed_authors":["Kawaguchi M","Suganuma N","Sato S","Shibata S","Hakoyama T","Umehara Y","Kouchi H","Yamaya-Ito H","Kaneko T","Hayashi M","Shimoda Y"],"additional_accession":[]},"is_claimable":false,"name":"A mitochondrial metalloprotease FtsH4 is required for symbiotic nitrogen fixation in Lotus japonicus nodules.","description":"Symbiotic nitrogen fixation is a highly coordinated process involving legume plants and nitrogen-fixing bacteria known as rhizobia. In this study, we investigated a novel Fix<sup>-</sup> mutant of the model legume Lotus japonicus that develops root nodules with endosymbiotic rhizobia but fails in nitrogen fixation. Map-based cloning identified the causal gene encoding the filamentation temperature-sensitive H (FtsH) protein, designated as LjFtsH4. The LjFtsH4 gene was expressed in all plant organs without increased levels during nodulation. Subcellular localization revealed that LjFtsH4, fused with a fluorescent protein, localized in mitochondria. The Ljftsh4 mutant nodules showed signs of premature senescence, including symbiosome membrane collapse and bacteroid disintegration. Additional","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-07-15T08:08:18.41Z","creation":"2025-04-04T12:01:14.602Z"},"accession":"S-EPMC11554776","cross_references":{"pubmed":["39528551"],"doi":["10.1038/s41598-024-78295-5"]}}