{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["19(1)"],"submitter":["Costa V"],"funding":["5X1000"],"pubmed_abstract":["<h4>Background</h4>Osteoarthritis (OA) is a degenerative musculoskeletal disease that significantly impacts the quality of life. Currently, no validated biomarkers for early detection of OA are defined. The possibility of discovering OA biomarkers is the focus of this study.<h4>Methods</h4>Human primary OA synovial cells (SVs), isolated from discarded joint tissue of patients with Kellgren & Lawrence score (KL) < 3, (mild/moderate) and KL ≥ 3 (severe), were characterized by FACS analysis. Through qRT-PCR and ELISA assays the inflammation, fibrosis status and the different miRNAs expression has been investigated. The role of miR-203a-3p and its precursors were evaluated through gain and loss of function study, IL-1β synoviocytes treatments and methylation analysis of miR203a promoter. The q"],"journal":["Journal of orthopaedic surgery and research"],"pagination":["746"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11558974"],"repository":["biostudies-literature"],"pubmed_title":["MiR203a-3p as a potential biomarker for synovial pathology associated with osteoarthritis: a pilot study."],"pmcid":["PMC11558974"],"pubmed_authors":["Bellavia D","Alessandro R","Terrando S","Giavaresi G","Salvatore C","Costa V"],"additional_accession":[]},"is_claimable":false,"name":"MiR203a-3p as a potential biomarker for synovial pathology associated with osteoarthritis: a pilot study.","description":"<h4>Background</h4>Osteoarthritis (OA) is a degenerative musculoskeletal disease that significantly impacts the quality of life. Currently, no validated biomarkers for early detection of OA are defined. The possibility of discovering OA biomarkers is the focus of this study.<h4>Methods</h4>Human primary OA synovial cells (SVs), isolated from discarded joint tissue of patients with Kellgren & Lawrence score (KL) < 3, (mild/moderate) and KL ≥ 3 (severe), were characterized by FACS analysis. Through qRT-PCR and ELISA assays the inflammation, fibrosis status and the different miRNAs expression has been investigated. The role of miR-203a-3p and its precursors were evaluated through gain and loss of function study, IL-1β synoviocytes treatments and methylation analysis of miR203a promoter. The q","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-07-16T19:33:21.426Z","creation":"2025-04-06T22:15:51.059Z"},"accession":"S-EPMC11558974","cross_references":{"pubmed":["39533317"],"doi":["10.1186/s13018-024-05237-2"]}}