{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tranchant EE"],"funding":["Københavns Universitet","Danmarks Frie Forskningsfond","Novo Nordisk Fonden","Lundbeck Foundation"],"pagination":["3759-3770"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11560309"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["123(21)"],"pubmed_abstract":["Measuring the compaction of a protein or complex is key to our understanding of the interactions within and between biomolecules. Experimentally, protein compaction is often probed either by estimating the radius of gyration (R<sub>g</sub>) obtained from small-angle x-ray scattering (SAXS) experiments or the hydrodynamic radius (R<sub>h</sub>) obtained, for example, by pulsed field gradient NMR (PFG NMR) spectroscopy. PFG NMR experiments generally report on the translational diffusion coefficient, which in turn can be used to estimate R<sub>h</sub> using an internal standard to account for sample viscosity and uncertainty about the gradient strength. 1,4-Dioxane is one such commonly used internal standard, and the reference value of R<sub>h</sub> is therefore important. We have revisited t"],"journal":["Biophysical journal"],"pubmed_title":["On the use of dioxane as reference for determination of the hydrodynamic radius by NMR spectroscopy."],"pmcid":["PMC11560309"],"funding_grant_id":["R155-2015-2666","9040-00164B","NNF18OC0033950","NNF18OC0033926","NNF19OC0055857"],"pubmed_authors":["Lindorff-Larsen K","Kragelund BB","Fernandes CB","Pesce F","Tranchant EE","Jacobsen NL"],"additional_accession":[]},"is_claimable":false,"name":"On the use of dioxane as reference for determination of the hydrodynamic radius by NMR spectroscopy.","description":"Measuring the compaction of a protein or complex is key to our understanding of the interactions within and between biomolecules. Experimentally, protein compaction is often probed either by estimating the radius of gyration (R<sub>g</sub>) obtained from small-angle x-ray scattering (SAXS) experiments or the hydrodynamic radius (R<sub>h</sub>) obtained, for example, by pulsed field gradient NMR (PFG NMR) spectroscopy. PFG NMR experiments generally report on the translational diffusion coefficient, which in turn can be used to estimate R<sub>h</sub> using an internal standard to account for sample viscosity and uncertainty about the gradient strength. 1,4-Dioxane is one such commonly used internal standard, and the reference value of R<sub>h</sub> is therefore important. We have revisited t","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-05T11:49:32.838Z","creation":"2026-05-16T03:13:31.663Z"},"accession":"S-EPMC11560309","cross_references":{"pubmed":["39340154"],"doi":["10.1016/j.bpj.2024.09.025"]}}