biostudies-literatureTranchant EEKøbenhavns UniversitetDanmarks Frie ForskningsfondNovo Nordisk FondenLundbeck Foundation3759-3770https://www.ebi.ac.uk/biostudies/studies/S-EPMC11560309biostudies-literatureUnknown123(21)Measuring the compaction of a protein or complex is key to our understanding of the interactions within and between biomolecules. Experimentally, protein compaction is often probed either by estimating the radius of gyration (R_g) obtained from small-angle x-ray scattering (SAXS) experiments or the hydrodynamic radius (R_h) obtained, for example, by pulsed field gradient NMR (PFG NMR) spectroscopy. PFG NMR experiments generally report on the translational diffusion coefficient, which in turn can be used to estimate R_h using an internal standard to account for sample viscosity and uncertainty about the gradient strength. 1,4-Dioxane is one such commonly used internal standard, and the reference value of R_h is therefore important. We have revisited tBiophysical journalOn the use of dioxane as reference for determination of the hydrodynamic radius by NMR spectroscopy.PMC11560309R155-2015-26669040-00164BNNF18OC0033950NNF18OC0033926NNF19OC0055857Lindorff-Larsen KKragelund BBFernandes CBPesce FTranchant EEJacobsen NLfalseOn the use of dioxane as reference for determination of the hydrodynamic radius by NMR spectroscopy.Measuring the compaction of a protein or complex is key to our understanding of the interactions within and between biomolecules. Experimentally, protein compaction is often probed either by estimating the radius of gyration (R_g) obtained from small-angle x-ray scattering (SAXS) experiments or the hydrodynamic radius (R_h) obtained, for example, by pulsed field gradient NMR (PFG NMR) spectroscopy. PFG NMR experiments generally report on the translational diffusion coefficient, which in turn can be used to estimate R_h using an internal standard to account for sample viscosity and uncertainty about the gradient strength. 1,4-Dioxane is one such commonly used internal standard, and the reference value of R_h is therefore important. We have revisited t2024-01-01T00:00:00Z2024 Nov2026-06-05T11:49:32.838Z2026-05-16T03:13:31.663ZS-EPMC115603093934015410.1016/j.bpj.2024.09.025