biostudies-literatureZhou YExcellent Young Scientists FundResearch Grants Council, University Grants CommitteeResearch Grants Council, University Grants Committee ()National Natural Science Foundation of ChinaExcellent Young Scientists Fund (Young Scientists Fund)China Postdoctoral Science FoundationNational Natural Science Foundation of China (NSFC)China Postdoctoral Science Foundation (China Postdoctoral Foundation Project)3834-3847https://www.ebi.ac.uk/biostudies/studies/S-EPMC11565167biostudies-literatureUnknown84(22)A variety of state-of-the-art nanovaccines (NV) combined with immunotherapies have recently been developed to treat malignant tumors, showing promising results. However, immunosuppression in the tumor microenvironment (TME) restrains cytotoxic T-cell infiltration and limits the efficacy of immunotherapies in solid tumors. Therefore, tactics for enhancing antigen cross-presentation and reshaping the TME need to be explored to enhance the activity of NVs. Here, we developed photo-enhanceable inflammasome-activating NVs (PIN) to achieve precise in situ delivery of a tumor antigen and a hydrophobic small molecule activating the nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain-containing protein 3 inflammasome (NLRP3) pathway. Near-infrared light irradiation promCancer researchPrecise In Situ Delivery of a Photo-Enhanceable Inflammasome-Activating Nanovaccine Activates Anticancer Immunity.PMC115651672023M734036822229032022TQ0388T12-703/19RLiu JWu MChen KDing TPang LWang WZhou YMan KfalsePrecise In Situ Delivery of a Photo-Enhanceable Inflammasome-Activating Nanovaccine Activates Anticancer Immunity.A variety of state-of-the-art nanovaccines (NV) combined with immunotherapies have recently been developed to treat malignant tumors, showing promising results. However, immunosuppression in the tumor microenvironment (TME) restrains cytotoxic T-cell infiltration and limits the efficacy of immunotherapies in solid tumors. Therefore, tactics for enhancing antigen cross-presentation and reshaping the TME need to be explored to enhance the activity of NVs. Here, we developed photo-enhanceable inflammasome-activating NVs (PIN) to achieve precise in situ delivery of a tumor antigen and a hydrophobic small molecule activating the nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain-containing protein 3 inflammasome (NLRP3) pathway. Near-infrared light irradiation prom2024-01-01T00:00:00Z2024 Nov2026-06-02T03:37:07.657Z2025-04-04T12:01:44.734ZS-EPMC115651673928807410.1158/0008-5472.CAN-24-0220