{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kohama T"],"funding":["JSPS KAKENHI","Japan Society for the Promotion of Science"],"pagination":["1156-1167"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11565231"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["111(5)"],"pubmed_abstract":["In vitro culture of ungrown oocytes in preantral follicles is one of the intriguing subjects being pursued to produce viable eggs in assisted reproductive technology. Previous studies have succeeded in obtaining mature eggs after in vitro culture of preantral follicles, while denuded undeveloped oocytes, which are obtained occasionally when collecting preantral follicles, seem to be almost useless. Moreover, methods to culture them efficiently to produce viable eggs have not been established yet. The present study was conducted to demonstrate in vitro culture of mouse denuded undeveloped oocytes by reconstructing granulosa cell-oocyte complexes, and to analyze cellular communication in reconstructed granulosa cell-oocyte complexes. Single denuded undeveloped oocytes were aggregated with 1 "],"journal":["Biology of reproduction"],"pubmed_title":["In vitro production of viable eggs from undeveloped oocytes in mouse preantral follicles by reconstructing granulosa cell-oocyte complexes†."],"pmcid":["PMC11565231"],"funding_grant_id":["JP19H05242","JP23KJ1040","JP22K06041"],"pubmed_authors":["Morohaku K","Kohama T","Tomioka I"],"additional_accession":[]},"is_claimable":false,"name":"In vitro production of viable eggs from undeveloped oocytes in mouse preantral follicles by reconstructing granulosa cell-oocyte complexes†.","description":"In vitro culture of ungrown oocytes in preantral follicles is one of the intriguing subjects being pursued to produce viable eggs in assisted reproductive technology. Previous studies have succeeded in obtaining mature eggs after in vitro culture of preantral follicles, while denuded undeveloped oocytes, which are obtained occasionally when collecting preantral follicles, seem to be almost useless. Moreover, methods to culture them efficiently to produce viable eggs have not been established yet. The present study was conducted to demonstrate in vitro culture of mouse denuded undeveloped oocytes by reconstructing granulosa cell-oocyte complexes, and to analyze cellular communication in reconstructed granulosa cell-oocyte complexes. Single denuded undeveloped oocytes were aggregated with 1 ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-03T03:13:28.2Z","creation":"2025-04-06T22:43:23.797Z"},"accession":"S-EPMC11565231","cross_references":{"pubmed":["39237319"],"doi":["10.1093/biolre/ioae125"]}}