{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Chen ZJ"],"funding":["NIDDK NIH HHS","NHLBI NIH HHS","NHGRI NIH HHS"],"pubmed_abstract":["Human subcutaneous adipose tissue (SAT) contains a diverse array of cell-types; however, the epigenomic landscape among the SAT cell-types has remained elusive. Our integrative analysis of single-cell resolution DNA methylation and chromatin conformation profiles (snm3C-seq), coupled with matching RNA expression (snRNA-seq), systematically cataloged the epigenomic, 3D topology, and transcriptomic dynamics across the SAT cell-types. We discovered that the SAT CG methylation (mCG) landscape is characterized by pronounced hyper-methylation in myeloid cells and hypo-methylation in adipocytes and adipose stem and progenitor cells (ASPCs), driving nearly half of the 705,063 detected differentially methylated regions (DMRs). In addition to the enriched cell-type-specific transcription factor bind"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.11.02.621694"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11566006"],"repository":["biostudies-literature"],"pubmed_title":["Single-cell DNA methylome and 3D genome atlas of the human subcutaneous adipose tissue."],"pmcid":["PMC11566006"],"funding_grant_id":["U01 HG012079","R01 DK132775","R01 HG010505","R01 HL170604"],"pubmed_authors":["Pietilainen KH","Alvarez M","Heinonen S","Peltoniemi H","Gelev KZ","Pajukanta P","Sankararaman S","Luo C","Kar A","Halperin E","Heffel MG","Zhang Y","Sukhatme MG","Chen ZJ","Lee SHT","Avram O","Rahmani E","Abuhanna KD","Das SS"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell DNA methylome and 3D genome atlas of the human subcutaneous adipose tissue.","description":"Human subcutaneous adipose tissue (SAT) contains a diverse array of cell-types; however, the epigenomic landscape among the SAT cell-types has remained elusive. Our integrative analysis of single-cell resolution DNA methylation and chromatin conformation profiles (snm3C-seq), coupled with matching RNA expression (snRNA-seq), systematically cataloged the epigenomic, 3D topology, and transcriptomic dynamics across the SAT cell-types. We discovered that the SAT CG methylation (mCG) landscape is characterized by pronounced hyper-methylation in myeloid cells and hypo-methylation in adipocytes and adipose stem and progenitor cells (ASPCs), driving nearly half of the 705,063 detected differentially methylated regions (DMRs). In addition to the enriched cell-type-specific transcription factor bind","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-04-25T03:28:03.834Z","creation":"2025-04-04T02:12:24.855Z"},"accession":"S-EPMC11566006","cross_references":{"pubmed":["39554055"],"doi":["10.1101/2024.11.02.621694"]}}