biostudies-literatureUnknownChen ZJNIDDK NIH HHSNHLBI NIH HHSNHGRI NIH HHSHuman subcutaneous adipose tissue (SAT) contains a diverse array of cell-types; however, the epigenomic landscape among the SAT cell-types has remained elusive. Our integrative analysis of single-cell resolution DNA methylation and chromatin conformation profiles (snm3C-seq), coupled with matching RNA expression (snRNA-seq), systematically cataloged the epigenomic, 3D topology, and transcriptomic dynamics across the SAT cell-types. We discovered that the SAT CG methylation (mCG) landscape is characterized by pronounced hyper-methylation in myeloid cells and hypo-methylation in adipocytes and adipose stem and progenitor cells (ASPCs), driving nearly half of the 705,063 detected differentially methylated regions (DMRs). In addition to the enriched cell-type-specific transcription factor bindbioRxiv : the preprint server for biology2024.11.02.621694https://www.ebi.ac.uk/biostudies/studies/S-EPMC11566006biostudies-literatureSingle-cell DNA methylome and 3D genome atlas of the human subcutaneous adipose tissue.PMC11566006U01 HG012079R01 DK132775R01 HG010505R01 HL170604Pietilainen KHAlvarez MHeinonen SPeltoniemi HGelev KZPajukanta PSankararaman SLuo CKar AHalperin EHeffel MGZhang YSukhatme MGChen ZJLee SHTAvram ORahmani EAbuhanna KDDas SSfalseSingle-cell DNA methylome and 3D genome atlas of the human subcutaneous adipose tissue.Human subcutaneous adipose tissue (SAT) contains a diverse array of cell-types; however, the epigenomic landscape among the SAT cell-types has remained elusive. Our integrative analysis of single-cell resolution DNA methylation and chromatin conformation profiles (snm3C-seq), coupled with matching RNA expression (snRNA-seq), systematically cataloged the epigenomic, 3D topology, and transcriptomic dynamics across the SAT cell-types. We discovered that the SAT CG methylation (mCG) landscape is characterized by pronounced hyper-methylation in myeloid cells and hypo-methylation in adipocytes and adipose stem and progenitor cells (ASPCs), driving nearly half of the 705,063 detected differentially methylated regions (DMRs). In addition to the enriched cell-type-specific transcription factor bind2024-01-01T00:00:00Z2024 Nov2026-04-25T03:28:03.834Z2025-04-04T02:12:24.855ZS-EPMC115660063955405510.1101/2024.11.02.621694