{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Stewart MG"],"funding":["NHGRI NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Cells coordinate diverse events at anaphase onset, including separase activation, cohesin cleavage, chromosome separation, and spindle reorganization. Regulation of the XMAP215 family member and microtubule polymerase, Stu2, at the metaphase-anaphase transition determines a specific redistribution from kinetochores to spindle microtubules. We show that cells modulate Stu2 kinetochore-microtubule localization by Polo-like kinase1/Cdc5-mediated phosphorylation of T866, near the Stu2 C-terminus, thereby promoting dissociation from the kinetochore Ndc80 complex. Cdk/Cdc28 likely primes Cdc5:Stu2 interaction. Cdc28 activity is also required for Stu2 nuclear import. PP2A<sup>Cdc55</sup> actively opposes Cdc5 activity on Stu2<sup>T866</sup> during metaphase. This counter-regulation allows for swi"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.11.01.621564"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11566010"],"repository":["biostudies-literature"],"pubmed_title":["A coordinated kinase and phosphatase network regulates Stu2 recruitment to yeast kinetochores."],"pmcid":["PMC11566010"],"funding_grant_id":["F31 CA271740","R35 GM142749","P30 CA015704","T32 HG008962"],"pubmed_authors":["Harrison SC","Carrier JS","Zahm JA","Stewart MG","Miller MP"],"additional_accession":[]},"is_claimable":false,"name":"A coordinated kinase and phosphatase network regulates Stu2 recruitment to yeast kinetochores.","description":"Cells coordinate diverse events at anaphase onset, including separase activation, cohesin cleavage, chromosome separation, and spindle reorganization. Regulation of the XMAP215 family member and microtubule polymerase, Stu2, at the metaphase-anaphase transition determines a specific redistribution from kinetochores to spindle microtubules. We show that cells modulate Stu2 kinetochore-microtubule localization by Polo-like kinase1/Cdc5-mediated phosphorylation of T866, near the Stu2 C-terminus, thereby promoting dissociation from the kinetochore Ndc80 complex. Cdk/Cdc28 likely primes Cdc5:Stu2 interaction. Cdc28 activity is also required for Stu2 nuclear import. PP2A<sup>Cdc55</sup> actively opposes Cdc5 activity on Stu2<sup>T866</sup> during metaphase. This counter-regulation allows for swi","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-04-08T18:44:25.077Z","creation":"2025-04-06T22:46:12.776Z"},"accession":"S-EPMC11566010","cross_references":{"pubmed":["39553986"],"doi":["10.1101/2024.11.01.621564"]}}