biostudies-literatureUnknownStewart MGNHGRI NIH HHSNCI NIH HHSNIGMS NIH HHSCells coordinate diverse events at anaphase onset, including separase activation, cohesin cleavage, chromosome separation, and spindle reorganization. Regulation of the XMAP215 family member and microtubule polymerase, Stu2, at the metaphase-anaphase transition determines a specific redistribution from kinetochores to spindle microtubules. We show that cells modulate Stu2 kinetochore-microtubule localization by Polo-like kinase1/Cdc5-mediated phosphorylation of T866, near the Stu2 C-terminus, thereby promoting dissociation from the kinetochore Ndc80 complex. Cdk/Cdc28 likely primes Cdc5:Stu2 interaction. Cdc28 activity is also required for Stu2 nuclear import. PP2A^Cdc55 actively opposes Cdc5 activity on Stu2^T866 during metaphase. This counter-regulation allows for swibioRxiv : the preprint server for biology2024.11.01.621564https://www.ebi.ac.uk/biostudies/studies/S-EPMC11566010biostudies-literatureA coordinated kinase and phosphatase network regulates Stu2 recruitment to yeast kinetochores.PMC11566010F31 CA271740R35 GM142749P30 CA015704T32 HG008962Harrison SCCarrier JSZahm JAStewart MGMiller MPfalseA coordinated kinase and phosphatase network regulates Stu2 recruitment to yeast kinetochores.Cells coordinate diverse events at anaphase onset, including separase activation, cohesin cleavage, chromosome separation, and spindle reorganization. Regulation of the XMAP215 family member and microtubule polymerase, Stu2, at the metaphase-anaphase transition determines a specific redistribution from kinetochores to spindle microtubules. We show that cells modulate Stu2 kinetochore-microtubule localization by Polo-like kinase1/Cdc5-mediated phosphorylation of T866, near the Stu2 C-terminus, thereby promoting dissociation from the kinetochore Ndc80 complex. Cdk/Cdc28 likely primes Cdc5:Stu2 interaction. Cdc28 activity is also required for Stu2 nuclear import. PP2A^Cdc55 actively opposes Cdc5 activity on Stu2^T866 during metaphase. This counter-regulation allows for swi2024-01-01T00:00:00Z2024 Nov2026-04-08T18:44:25.077Z2025-04-06T22:46:12.776ZS-EPMC115660103955398610.1101/2024.11.01.621564