biostudies-literatureUnknown19(1)Andersen KDanmarks Frie ForskningsfondKræftens BekæmpelseTorben og Alice Frimodts FondNovo Nordisk FondenAase og Ejnar Danielsens Fond<h4>Background</h4>Human papillomavirus (HPV) has been proposed to contribute to the carcinogenesis of prostate cancer. However, previous studies have yielded conflicting results. This study aims to add useful information to the ongoing discussion concerning the association between HPV infection and prostate cancer.<h4>Methods</h4>We used two high-throughput next-generation sequencing (NGS) approaches to detect HPV RNA in malignant and adjacent normal (AN) prostate tissue (cohorts 1 and 2) and HPV DNA from carcinogenic and probably/possibly carcinogenic-classified HPV types (cohort 3) in malignant prostate, AN prostate, and benign prostatic hyperplasia (BPH) tissues.<h4>Results</h4>In total, 0% (cohort 1: 0/83, cohort 2: 0/16) of the malignant prostate tissue samples and 0% (cohort 1: 0/23Infectious agents and cancer57https://www.ebi.ac.uk/biostudies/studies/S-EPMC11566718biostudies-literatureHighly sensitive deep panel sequencing of 27 HPV genotypes in prostate cancer biopsies results in very low detection rates and indicates that HPV is not a major etiological driver of this malignancy.PMC11566718Steiniche TSalachan PVBorre MSorensen KDAndersen KUlhoi BStougaard MfalseHighly sensitive deep panel sequencing of 27 HPV genotypes in prostate cancer biopsies results in very low detection rates and indicates that HPV is not a major etiological driver of this malignancy.<h4>Background</h4>Human papillomavirus (HPV) has been proposed to contribute to the carcinogenesis of prostate cancer. However, previous studies have yielded conflicting results. This study aims to add useful information to the ongoing discussion concerning the association between HPV infection and prostate cancer.<h4>Methods</h4>We used two high-throughput next-generation sequencing (NGS) approaches to detect HPV RNA in malignant and adjacent normal (AN) prostate tissue (cohorts 1 and 2) and HPV DNA from carcinogenic and probably/possibly carcinogenic-classified HPV types (cohort 3) in malignant prostate, AN prostate, and benign prostatic hyperplasia (BPH) tissues.<h4>Results</h4>In total, 0% (cohort 1: 0/83, cohort 2: 0/16) of the malignant prostate tissue samples and 0% (cohort 1: 0/232024-01-01T00:00:00Z2024 Nov2026-07-16T22:34:37.168Z2025-04-05T18:39:30.522ZS-EPMC115667183954373810.1186/s13027-024-00619-x