biostudies-literaturePanpetch WProgram Management Unit for Human Resources & Institutional Development, Research and InnovationRachadapisek Sompote Fund for Postdoctoral Fellowship, Chulalongkorn UniversityNational Research Council of ThailandThailand Science Research and Innovation Fund Chulalongkorn UniversityRachadapisek Sompote Matching Funde0309106https://www.ebi.ac.uk/biostudies/studies/S-EPMC11567622biostudies-literatureUnknown19(11)The impact of Pseudomonas aeruginosa (PA) was explored in a mouse model with non-diarrheal gut permeability defect using 1.5% dextran sulfate solution (DSS) plus antibiotics (ATB) with or without orally administered PA. As such, ATB+DSS+PA mice induced more severe intestinal injury as indicated by stool consistency and leaky gut (FITC-dextran assay, bacteremia, and endotoxemia) with an increase in serum cytokines, liver enzyme, and hepatocyte apoptosis when compared with ATB+DSS mice. There was no abnormality by these parameters in the non-DSS group, including water alone (control), antibiotics alone (ATB+water), and antibiotics with PA (ATB+water+PA). Despite a similarly fecal microbiome patterns between ATB+DSS and ATB+DSS+PA groups, a higher abundance of Pseudomonas, Enterococci, and EsPloS onePresence of Pseudomonas aeruginosa in feces exacerbate leaky gut in mice with low dose dextran sulfate solution, impacts of specific bacteria.PMC11567622B16F640175 and B48G660112HEAF67300087RA-MF-22/65, RA-MF-13/66, and RA-MF-eAsiaNRCT-N41A640076 and NRCT-N34A660583Tumwasorn SPanpetch WLeelahavanichkul AfalsePresence of Pseudomonas aeruginosa in feces exacerbate leaky gut in mice with low dose dextran sulfate solution, impacts of specific bacteria.The impact of Pseudomonas aeruginosa (PA) was explored in a mouse model with non-diarrheal gut permeability defect using 1.5% dextran sulfate solution (DSS) plus antibiotics (ATB) with or without orally administered PA. As such, ATB+DSS+PA mice induced more severe intestinal injury as indicated by stool consistency and leaky gut (FITC-dextran assay, bacteremia, and endotoxemia) with an increase in serum cytokines, liver enzyme, and hepatocyte apoptosis when compared with ATB+DSS mice. There was no abnormality by these parameters in the non-DSS group, including water alone (control), antibiotics alone (ATB+water), and antibiotics with PA (ATB+water+PA). Despite a similarly fecal microbiome patterns between ATB+DSS and ATB+DSS+PA groups, a higher abundance of Pseudomonas, Enterococci, and Es2024-01-01T00:00:00Z20242026-06-01T07:18:27.784Z2025-04-06T22:46:23.056ZS-EPMC115676223954643510.1371/journal.pone.0309106