{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhao AY"],"funding":["NCATS NIH HHS","National Heart, Lung, and Blood Institute","NHLBI NIH HHS","NLM NIH HHS","Pulmonary Fibrosis Foundation","National Institute of General Medical Sciences","Three Lakes Foundation","Veracyte","NIGMS NIH HHS"],"pagination":["1252-1266"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11568434"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["210(10)"],"pubmed_abstract":["<b>Rationale:</b> Fibrotic hypersensitivity pneumonitis (FHP) is a debilitating interstitial lung disease driven by incompletely understood immune mechanisms. <b>Objectives:</b> To elucidate immune aberrations in FHP in single-cell resolution. <b>Methods:</b> Single-cell 5' RNA sequencing was conducted on peripheral blood mononuclear cells and BAL cells obtained from 45 patients with FHP, 63 patients with idiopathic pulmonary fibrosis (IPF), 4 patients with nonfibrotic hypersensitivity pneumonitis, and 36 healthy control subjects in the United States and Mexico. Analyses included differential gene expression (Seurat), TF (transcription factor) activity imputation (DoRothEA-VIPER), and trajectory analyses (Monocle3 and Velocyto-scVelo-CellRank). <b>Measurements and Main Results:</b> Overall"],"journal":["American journal of respiratory and critical care medicine"],"pubmed_title":["Single-Cell Analysis Reveals Novel Immune Perturbations in Fibrotic Hypersensitivity Pneumonitis."],"pmcid":["PMC11568434"],"funding_grant_id":["T32GM086287","U01 HL145567","R21 LM012884","R01 HL152677","UL1 TR001863","K08 HL151970","1K08HL151970-01","R21 HL161723","R01 HL127349","R01 HL163984","R01 LM014087","K23 HL146942","R01 HL141852","T32 GM086287","F30 HL162459"],"pubmed_authors":["Justet A","Pardo A","Deluliis G","Flint J","Buendia-Roldan I","Adegunsoye A","Zhao J","Selman M","Sumida TS","Kaminski N","Abu Hussein NS","Raredon MSB","Sharma P","Ahangari F","Unterman A","Yan X","Adams TS","Schupp JC","Ryu C","Zhao AY","Sperling AI","Prasse A","Nikola F","Zhang Y","Herzog E"],"additional_accession":[]},"is_claimable":false,"name":"Single-Cell Analysis Reveals Novel Immune Perturbations in Fibrotic Hypersensitivity Pneumonitis.","description":"<b>Rationale:</b> Fibrotic hypersensitivity pneumonitis (FHP) is a debilitating interstitial lung disease driven by incompletely understood immune mechanisms. <b>Objectives:</b> To elucidate immune aberrations in FHP in single-cell resolution. <b>Methods:</b> Single-cell 5' RNA sequencing was conducted on peripheral blood mononuclear cells and BAL cells obtained from 45 patients with FHP, 63 patients with idiopathic pulmonary fibrosis (IPF), 4 patients with nonfibrotic hypersensitivity pneumonitis, and 36 healthy control subjects in the United States and Mexico. Analyses included differential gene expression (Seurat), TF (transcription factor) activity imputation (DoRothEA-VIPER), and trajectory analyses (Monocle3 and Velocyto-scVelo-CellRank). <b>Measurements and Main Results:</b> Overall","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-03T03:12:06.257Z","creation":"2025-04-06T22:45:51.734Z"},"accession":"S-EPMC11568434","cross_references":{"pubmed":["38924775"],"doi":["10.1164/rccm.202401-0078OC"]}}