biostudies-literatureZhao AYNCATS NIH HHSNational Heart, Lung, and Blood InstituteNHLBI NIH HHSNLM NIH HHSPulmonary Fibrosis FoundationNational Institute of General Medical SciencesThree Lakes FoundationVeracyteNIGMS NIH HHS1252-1266https://www.ebi.ac.uk/biostudies/studies/S-EPMC11568434biostudies-literatureUnknown210(10)<b>Rationale:</b> Fibrotic hypersensitivity pneumonitis (FHP) is a debilitating interstitial lung disease driven by incompletely understood immune mechanisms. <b>Objectives:</b> To elucidate immune aberrations in FHP in single-cell resolution. <b>Methods:</b> Single-cell 5' RNA sequencing was conducted on peripheral blood mononuclear cells and BAL cells obtained from 45 patients with FHP, 63 patients with idiopathic pulmonary fibrosis (IPF), 4 patients with nonfibrotic hypersensitivity pneumonitis, and 36 healthy control subjects in the United States and Mexico. Analyses included differential gene expression (Seurat), TF (transcription factor) activity imputation (DoRothEA-VIPER), and trajectory analyses (Monocle3 and Velocyto-scVelo-CellRank). <b>Measurements and Main Results:</b> OverallAmerican journal of respiratory and critical care medicineSingle-Cell Analysis Reveals Novel Immune Perturbations in Fibrotic Hypersensitivity Pneumonitis.PMC11568434T32GM086287U01 HL145567R21 LM012884R01 HL152677UL1 TR001863K08 HL1519701K08HL151970-01R21 HL161723R01 HL127349R01 HL163984R01 LM014087K23 HL146942R01 HL141852T32 GM086287F30 HL162459Justet APardo ADeluliis GFlint JBuendia-Roldan IAdegunsoye AZhao JSelman MSumida TSKaminski NAbu Hussein NSRaredon MSBSharma PAhangari FUnterman AYan XAdams TSSchupp JCRyu CZhao AYSperling AIPrasse ANikola FZhang YHerzog EfalseSingle-Cell Analysis Reveals Novel Immune Perturbations in Fibrotic Hypersensitivity Pneumonitis.<b>Rationale:</b> Fibrotic hypersensitivity pneumonitis (FHP) is a debilitating interstitial lung disease driven by incompletely understood immune mechanisms. <b>Objectives:</b> To elucidate immune aberrations in FHP in single-cell resolution. <b>Methods:</b> Single-cell 5' RNA sequencing was conducted on peripheral blood mononuclear cells and BAL cells obtained from 45 patients with FHP, 63 patients with idiopathic pulmonary fibrosis (IPF), 4 patients with nonfibrotic hypersensitivity pneumonitis, and 36 healthy control subjects in the United States and Mexico. Analyses included differential gene expression (Seurat), TF (transcription factor) activity imputation (DoRothEA-VIPER), and trajectory analyses (Monocle3 and Velocyto-scVelo-CellRank). <b>Measurements and Main Results:</b> Overall2024-01-01T00:00:00Z2024 Nov2026-06-03T03:12:06.257Z2025-04-06T22:45:51.734ZS-EPMC115684343892477510.1164/rccm.202401-0078OC