biostudies-literatureUnknown22(1)Zhong S<h4>Background</h4>Liver organoid serves as an alternative model for liver pathophysiology in carbohydrate or lipid metabolism and xenobiotic metabolism transformation. Biomechanical cues including spaceflight mission can affect liver organoid construction and their related functions, but their underlying mechanisms are not fully understood yet. Here, a rotating cell culture device, namely Rotating Flat Chamber (RFC), was specifically designed for adhering cells or cell aggregated to elucidate the effects of altered gravity vector on HepaRG-derived liver organoids construction.<h4>Results</h4>The organoids so formed under RFC presented the fast growth rate and large projection area. Meanwhile, the expressions of two pluripotency markers of SOX9 and CD44 were enhanced. This finding was posiBMC biology262https://www.ebi.ac.uk/biostudies/studies/S-EPMC11568593biostudies-literatureRotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation.PMC11568593Luo QZhong SZheng LSun SLu DLong MSong GWu YfalseRotating culture regulates the formation of HepaRG-derived liver organoids via YAP translocation.<h4>Background</h4>Liver organoid serves as an alternative model for liver pathophysiology in carbohydrate or lipid metabolism and xenobiotic metabolism transformation. Biomechanical cues including spaceflight mission can affect liver organoid construction and their related functions, but their underlying mechanisms are not fully understood yet. Here, a rotating cell culture device, namely Rotating Flat Chamber (RFC), was specifically designed for adhering cells or cell aggregated to elucidate the effects of altered gravity vector on HepaRG-derived liver organoids construction.<h4>Results</h4>The organoids so formed under RFC presented the fast growth rate and large projection area. Meanwhile, the expressions of two pluripotency markers of SOX9 and CD44 were enhanced. This finding was posi2024-01-01T00:00:00Z2024 Nov2026-06-01T07:16:54.93Z2025-04-19T09:59:40.958ZS-EPMC115685933954850910.1186/s12915-024-02062-1