biostudies-literatureUnknownWatanabe SChiba Foundation for Health Promotion and Disease PreventionThe Japanese Orthopaedic Society of Knee, Arthroscopy and Sports MedicineJapan Science and Technology AgencyJapan Society for the Promotion of Science<h4>Objective</h4>Multilineage differentiating stress-enduring (Muse) cells, a pluripotent stem cell subset of mesenchymal stem cells (MSCs), have shown promise for various tissue repairs due to their stress tolerance and multipotent capabilities. We aimed to investigate the differentiation potential <i>in vitro</i>, the dynamics <i>in vivo</i>, and the reparative contribution of Muse cells to osteochondral lesions.<h4>Design</h4>Labeled MSCs were cultured and sorted into Muse and non-Muse (MSCs without Muse cells) groups. These cells were then formed into spheroids, and chondrogenic differentiation was assessed <i>in vitro</i>. Twenty-one immunocompromised mice were used as the <i>in vivo</i> models of osteochondral lesions. Live imaging, macroscopic evaluation, and histological and immunCartilage19476035241262020https://www.ebi.ac.uk/biostudies/studies/S-EPMC11569652biostudies-literatureInvestigating the Potential of Multilineage Differentiating Stress-Enduring Cells for Osteochondral Healing.PMC11569652JP22K16705JPMJSP2109Sasho TWatanabe SKimura SOno YYamaguchi SOhtori SHosokawa HHorii MSakamoto TfalseInvestigating the Potential of Multilineage Differentiating Stress-Enduring Cells for Osteochondral Healing.<h4>Objective</h4>Multilineage differentiating stress-enduring (Muse) cells, a pluripotent stem cell subset of mesenchymal stem cells (MSCs), have shown promise for various tissue repairs due to their stress tolerance and multipotent capabilities. We aimed to investigate the differentiation potential <i>in vitro</i>, the dynamics <i>in vivo</i>, and the reparative contribution of Muse cells to osteochondral lesions.<h4>Design</h4>Labeled MSCs were cultured and sorted into Muse and non-Muse (MSCs without Muse cells) groups. These cells were then formed into spheroids, and chondrogenic differentiation was assessed <i>in vitro</i>. Twenty-one immunocompromised mice were used as the <i>in vivo</i> models of osteochondral lesions. Live imaging, macroscopic evaluation, and histological and immun2024-01-01T00:00:00Z2024 Jun2025-04-04T00:48:07.934Z2025-04-04T00:48:07.934ZS-EPMC115696523888703810.1177/19476035241262020