biostudies-literatureMehra RNCI NIH HHSe2300565https://www.ebi.ac.uk/biostudies/studies/S-EPMC11569832biostudies-literatureUnknown8<h4>Purpose</h4>Develop and validate gene expression-based biomarker associated with recurrent disease to facilitate risk stratification of clear cell renal cell carcinoma (ccRCC).<h4>Materials and methods</h4>We retrospectively identified 110 patients who underwent radical nephrectomy for ccRCC (<i>discovery</i> cohort). Patients who recurred were matched on the basis of grade/stage to patients without recurrence. Capture whole-transcriptome sequencing was performed on RNA isolated from archival tissue using the Illumina platform. We developed a gene-expression signature to predict recurrence-free survival/disease-free survival (DFS) using a 15-fold lasso and elastic-net regularized linear Cox model. We derived the 31-gene cell cycle progression (mxCCP) score using RNA-seq data for each pJCO precision oncologyDiscovery and Validation of a 15-Gene Prognostic Signature for Clear Cell Renal Cell Carcinoma.PMC11569832P30 CA046592R37 CA283857Nallandhighal SKnuth ZSu FCotta BKasputis ACao XWang RZhang YCieslik MPSalami SSMehra RUdager AMDhanasekaran SMMorgan TMfalseDiscovery and Validation of a 15-Gene Prognostic Signature for Clear Cell Renal Cell Carcinoma.<h4>Purpose</h4>Develop and validate gene expression-based biomarker associated with recurrent disease to facilitate risk stratification of clear cell renal cell carcinoma (ccRCC).<h4>Materials and methods</h4>We retrospectively identified 110 patients who underwent radical nephrectomy for ccRCC (<i>discovery</i> cohort). Patients who recurred were matched on the basis of grade/stage to patients without recurrence. Capture whole-transcriptome sequencing was performed on RNA isolated from archival tissue using the Illumina platform. We developed a gene-expression signature to predict recurrence-free survival/disease-free survival (DFS) using a 15-fold lasso and elastic-net regularized linear Cox model. We derived the 31-gene cell cycle progression (mxCCP) score using RNA-seq data for each p2024-01-01T00:00:00Z2024 May2026-06-02T20:43:30.377Z2026-04-20T03:10:35.078ZS-EPMC115698323881017910.1200/PO.23.0056510.1200/po.23.00565