{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["86(11)"],"submitter":["Amer MM"],"funding":["Ministry of Higher Education and Scientific Research of the Arab Republic of Egypt"],"pubmed_abstract":["In Egypt, camel trypanosomiasis is widespread. From October 2021 to March 2022, we collected 181 blood samples from apparently healthy one-humped camels (Camelus dromedarius) in Cairo and Giza Governates. The objective of this study was to assess infection rates of trypanosomes using blood smear examination and PCR-sequencing assays. Trypanosomes were detected in 8.3% (15/181) of camels by blood smear and in 23.8% (43/181) by PCR targeting the internal transcribed spacer (ITS). Based on blood smear and ITS-PCR results, and the absence of tsetse flies in the study area, we hypothesized that the Trypanosoma species was likely T. evansi. Validation using PCR based on the variant surface glycoprotein (VSG) of T. evansi Rode Trypanozoon antigen type (RoTat) 1.2 (RoTat 1.2 VSG gene) on ITS-PCR-p"],"journal":["The Journal of veterinary medical science"],"pagination":["1177-1184"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11569872"],"repository":["biostudies-literature"],"pubmed_title":["Parasitological and molecular investigation of Trypanosoma evansi in dromedaries from Greater Cairo, Egypt."],"pmcid":["PMC11569872"],"pubmed_authors":["Li Y","Hegab AA","Jaroszewski J","Mohanta UK","Soliman AM","El-Kelesh EA","Xuan X","Amer MM","DO T"],"additional_accession":[]},"is_claimable":false,"name":"Parasitological and molecular investigation of Trypanosoma evansi in dromedaries from Greater Cairo, Egypt.","description":"In Egypt, camel trypanosomiasis is widespread. From October 2021 to March 2022, we collected 181 blood samples from apparently healthy one-humped camels (Camelus dromedarius) in Cairo and Giza Governates. The objective of this study was to assess infection rates of trypanosomes using blood smear examination and PCR-sequencing assays. Trypanosomes were detected in 8.3% (15/181) of camels by blood smear and in 23.8% (43/181) by PCR targeting the internal transcribed spacer (ITS). Based on blood smear and ITS-PCR results, and the absence of tsetse flies in the study area, we hypothesized that the Trypanosoma species was likely T. evansi. Validation using PCR based on the variant surface glycoprotein (VSG) of T. evansi Rode Trypanozoon antigen type (RoTat) 1.2 (RoTat 1.2 VSG gene) on ITS-PCR-p","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-07-16T23:55:59.253Z","creation":"2025-04-03T23:23:54.418Z"},"accession":"S-EPMC11569872","cross_references":{"pubmed":["39358244"],"doi":["10.1292/jvms.24-0284"]}}