biostudies-literatureMandalasi MNNational Institute of Allergy and Infectious DiseasesNational Institute of Allergy and Infectious Diseases Division of Intramural ResearchNIAID NIH HHSNational Institutes of Health107801https://www.ebi.ac.uk/biostudies/studies/S-EPMC11570480biostudies-literatureUnknown300(11)A dynamic proteome is required for cellular adaption to changing environments including levels of O₂, and the SKP1/CULLIN-1/F-box protein/RBX1 (SCF) family of E3 ubiquitin ligases contributes importantly to proteasome-mediated degradation. We examine, in the apicomplexan parasite Toxoplasma gondii, the influence on the interactome of SKP1 by its novel glycan attached to hydroxyproline generated by PHYa, the likely ortholog of the HIFα PHD2 oxygen-sensor of human host cells. Strikingly, the representation of several putative F-box proteins (FBPs) is substantially reduced in PHYaΔ parasites grown in fibroblasts. One, FBXO13, is a predicted lysyl hydroxylase related to the human JmjD6 oncogene except for its F-box domain. The abundance of FBXO13, epitope-tagged at its genetic locusThe Journal of biological chemistryOxygen-dependent regulation of F-box proteins in Toxoplasma gondii is mediated by Skp1 glycosylation.PMC11570480R01 AI150240R01-AI150240R01 AI169849R21-AI149405T32 AI060546T32-AI060546R21 AI149405Deng BMandalasi MNBoons GJBaptista CGWest CMGas-Pascual EKruijtzer JAWvan der Wel HBlader IJfalseOxygen-dependent regulation of F-box proteins in Toxoplasma gondii is mediated by Skp1 glycosylation.A dynamic proteome is required for cellular adaption to changing environments including levels of O₂, and the SKP1/CULLIN-1/F-box protein/RBX1 (SCF) family of E3 ubiquitin ligases contributes importantly to proteasome-mediated degradation. We examine, in the apicomplexan parasite Toxoplasma gondii, the influence on the interactome of SKP1 by its novel glycan attached to hydroxyproline generated by PHYa, the likely ortholog of the HIFα PHD2 oxygen-sensor of human host cells. Strikingly, the representation of several putative F-box proteins (FBPs) is substantially reduced in PHYaΔ parasites grown in fibroblasts. One, FBXO13, is a predicted lysyl hydroxylase related to the human JmjD6 oncogene except for its F-box domain. The abundance of FBXO13, epitope-tagged at its genetic locus2024-01-01T00:00:00Z2024 Nov2026-05-26T14:00:18.837Z2025-04-21T21:45:11.435ZS-EPMC115704803930730710.1016/j.jbc.2024.107801