{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(1)"],"submitter":["Liu W"],"pubmed_abstract":["Electrocatalytic carbon dioxide (CO<sub>2</sub>) reduction by CO<sub>2</sub> reductases is a promising approach for biomanufacturing. Among all known biological or chemical catalysts, hydrogen-dependent carbon dioxide reductase from Thermoanaerobacter kivui (TkHDCR) possesses the highest activity toward CO<sub>2</sub> reduction. Herein, we engineer TkHDCR to generate an electro-responsive carbon dioxide reductase considering the safety and convenience. To achieve this purpose, a recombinant Escherichia coli TkHDCR overexpression system is established. The formate dehydrogenase is obtained via subunit truncation and rational design, which enables direct electron transfer (DET)-type bioelectrocatalysis with a near-zero overpotential. By applying a constant voltage of -500 mV (vs. SHE) to a m"],"journal":["Nature communications"],"pagination":["9962"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11570645"],"repository":["biostudies-literature"],"pubmed_title":["Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui."],"pmcid":["PMC11570645"],"pubmed_authors":["Liu J","Zhang K","Cui H","Zhang M","Zhang L","Wang Y","Sun J","Liu W"],"additional_accession":[]},"is_claimable":false,"name":"Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui.","description":"Electrocatalytic carbon dioxide (CO<sub>2</sub>) reduction by CO<sub>2</sub> reductases is a promising approach for biomanufacturing. Among all known biological or chemical catalysts, hydrogen-dependent carbon dioxide reductase from Thermoanaerobacter kivui (TkHDCR) possesses the highest activity toward CO<sub>2</sub> reduction. Herein, we engineer TkHDCR to generate an electro-responsive carbon dioxide reductase considering the safety and convenience. To achieve this purpose, a recombinant Escherichia coli TkHDCR overexpression system is established. The formate dehydrogenase is obtained via subunit truncation and rational design, which enables direct electron transfer (DET)-type bioelectrocatalysis with a near-zero overpotential. By applying a constant voltage of -500 mV (vs. SHE) to a m","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2025-04-04T00:48:15.077Z","creation":"2025-04-04T00:48:15.077Z"},"accession":"S-EPMC11570645","cross_references":{"pubmed":["39551789"],"doi":["10.1038/s41467-024-53946-3"]}}