{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(1)"],"submitter":["Murphy KR"],"pubmed_abstract":["High-frequency irreversible electroporation (H-FIRE), a nonthermal brain tumor ablation therapeutic, generates a central tumor ablation zone while transiently disrupting the peritumoral blood-brain barrier (BBB). We hypothesized that bystander effects of H-FIRE tumor cell ablation, mediated by small tumor-derived extracellular vesicles (sTDEV), disrupt the BBB endothelium. Monolayers of bEnd.3 cerebral endothelial cells were exposed to supernatants of H-FIRE or radiation (RT)-treated LL/2 and F98 cancer cells. Endothelial cell response was evaluated microscopically and via flow cytometry for apoptosis. sTDEV were isolated following H-FIRE and RT, characterized via nanoparticle tracking analysis (NTA) and transmission electron microscopy, and applied to a Transwell BBB endothelium model to "],"journal":["Scientific reports"],"pagination":["28533"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11574144"],"repository":["biostudies-literature"],"pubmed_title":["Tumor-derived extracellular vesicles disrupt the blood-brain barrier endothelium following high-frequency irreversible electroporation."],"pmcid":["PMC11574144"],"pubmed_authors":["Aycock KN","Athanasiadi I","Bracha S","Marsh S","Murphy KR","Rossmeisl JH","Dervisis NG","Hay AN","Chang C","Gourdie R","Davalos RV"],"additional_accession":[]},"is_claimable":false,"name":"Tumor-derived extracellular vesicles disrupt the blood-brain barrier endothelium following high-frequency irreversible electroporation.","description":"High-frequency irreversible electroporation (H-FIRE), a nonthermal brain tumor ablation therapeutic, generates a central tumor ablation zone while transiently disrupting the peritumoral blood-brain barrier (BBB). We hypothesized that bystander effects of H-FIRE tumor cell ablation, mediated by small tumor-derived extracellular vesicles (sTDEV), disrupt the BBB endothelium. Monolayers of bEnd.3 cerebral endothelial cells were exposed to supernatants of H-FIRE or radiation (RT)-treated LL/2 and F98 cancer cells. Endothelial cell response was evaluated microscopically and via flow cytometry for apoptosis. sTDEV were isolated following H-FIRE and RT, characterized via nanoparticle tracking analysis (NTA) and transmission electron microscopy, and applied to a Transwell BBB endothelium model to ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-07-17T00:01:55.513Z","creation":"2025-04-05T19:58:41.22Z"},"accession":"S-EPMC11574144","cross_references":{"pubmed":["39557959"],"doi":["10.1038/s41598-024-79019-5"]}}