biostudies-literatureErickson KDHHS | National Institutes of HealthHHS | National Institutes of Health (NIH)NIAID NIH HHSNIH HHSe0079024https://www.ebi.ac.uk/biostudies/studies/S-EPMC11575236biostudies-literatureUnknown98(11)Polyomaviruses (PyVs) cause diverse diseases in a variety of mammalian hosts. During the life cycle, PyVs recruit nuclear host factors to viral genomes to facilitate replication and transcription. While host factors involved in DNA replication, DNA damage sensing and repair, and cell cycle regulation have been observed to bind PyV DNA, the complete set of viral and host proteins comprising the PyV replisome remains incompletely characterized. Here, the iPOND-MS technique (Isolation of Proteins on Nascent DNA coupled with Mass Spectrometry) was used to identify the proteome bound to murine PyV (MuPyV) DNA immediately following synthesis and 2 hours post-synthesis. Several novel MuPyV DNA interactors were identified on newly synthesized viral DNA (vDNA), including MCM complex members, DNA prJournal of virologyProteome profiling of polyomavirus nuclear replication centers using iPOND.PMC11575236S10 OD025072R01 AI151636R01 AI151636-37Holland ALangsfeld ESEbmeier CCGarcea RLErickson KDfalseProteome profiling of polyomavirus nuclear replication centers using iPOND.Polyomaviruses (PyVs) cause diverse diseases in a variety of mammalian hosts. During the life cycle, PyVs recruit nuclear host factors to viral genomes to facilitate replication and transcription. While host factors involved in DNA replication, DNA damage sensing and repair, and cell cycle regulation have been observed to bind PyV DNA, the complete set of viral and host proteins comprising the PyV replisome remains incompletely characterized. Here, the iPOND-MS technique (Isolation of Proteins on Nascent DNA coupled with Mass Spectrometry) was used to identify the proteome bound to murine PyV (MuPyV) DNA immediately following synthesis and 2 hours post-synthesis. Several novel MuPyV DNA interactors were identified on newly synthesized viral DNA (vDNA), including MCM complex members, DNA pr2024-01-01T00:00:00Z2024 Nov2026-07-17T01:07:48.564Z2025-04-04T20:38:43.355ZS-EPMC115752363948011010.1128/jvi.00790-24