{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Choi YG"],"funding":["NHLBI NIH HHS","U.S. Department of Defense","National Institutes of Health","NIH HHS"],"pagination":["28688"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11576751"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["ETV2 is an essential transcription factor as Etv2 null murine embryos lack all vasculature, blood and are lethal early during embryogenesis. Previous studies have established that ETV2 functions as a pioneer factor and directly reprograms fibroblasts to endothelial cells. However, the underlying molecular mechanisms regulating this reprogramming process remain incompletely defined. In the present study, we examined the ETV2-RIG1 cascade as regulators that govern ETV2-mediated reprogramming. Mouse embryonic fibroblasts (MEFs) harboring an inducible ETV2 expression system were used to overexpress ETV2 and reprogram these somatic cells to the endothelial lineage. Single-cell RNA-seq from reprogrammed fibroblasts defined the induction of the transcriptional network involved in Rig1-like recept"],"journal":["Scientific reports"],"pubmed_title":["ETV2 transcriptionally activates Rig1 gene expression and promotes reprogramming of the endothelial lineage."],"pmcid":["PMC11576751"],"funding_grant_id":["PR200679","R01 HL168647","HL148599","R01 HL148599","P01 HL160476","P01HL160476"],"pubmed_authors":["Sierra-Pagan JE","Zhang JJ","Garry DJ","Das S","Ma X","Choi YG","Garry MG","Sadek HA","Larson T","Gong W"],"additional_accession":[]},"is_claimable":false,"name":"ETV2 transcriptionally activates Rig1 gene expression and promotes reprogramming of the endothelial lineage.","description":"ETV2 is an essential transcription factor as Etv2 null murine embryos lack all vasculature, blood and are lethal early during embryogenesis. Previous studies have established that ETV2 functions as a pioneer factor and directly reprograms fibroblasts to endothelial cells. However, the underlying molecular mechanisms regulating this reprogramming process remain incompletely defined. In the present study, we examined the ETV2-RIG1 cascade as regulators that govern ETV2-mediated reprogramming. Mouse embryonic fibroblasts (MEFs) harboring an inducible ETV2 expression system were used to overexpress ETV2 and reprogram these somatic cells to the endothelial lineage. Single-cell RNA-seq from reprogrammed fibroblasts defined the induction of the transcriptional network involved in Rig1-like recept","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-02T09:21:00.742Z","creation":"2025-04-04T01:18:07.385Z"},"accession":"S-EPMC11576751","cross_references":{"pubmed":["39562637"],"doi":["10.1038/s41598-024-78115-w"]}}