biostudies-literatureLiu Mthe National Key Research and Development Program of ChinaScience and Technology Program of Shenyang28583https://www.ebi.ac.uk/biostudies/studies/S-EPMC11577115biostudies-literatureUnknown14(1)Phosphorus deficiency is a major influence on growth and development of soybean. Therefore, improving phosphorus utilization efficiency in soybean is a research priority for the soybean community. In this experiment, Liaodou 13 (high phosphorus utilization: HPE) and Tiefeng 3 (low phosphorus utilization: LPE) were used as test varieties. We investigated changes in root morphology, amino acid content, and content of key substances of the nitrogen metabolic pathway with normal phosphorus (0.5 mM) and low phosphorus (0.005 mM) treatments. The results showed that the root length, root surface area and number of lateral roots of HPE roots were higher than those of LPE roots under normal and low phosphorus conditions. The contents of different types of amino acids showed different trends in two Scientific reportsRoot morphology, nitrogen metabolism and amino acid metabolism in soybean under low phosphorus stress.PMC11577115No. 23-410-2-092022YFE0203300, 2018YFE0116900Yang GSun MZhao MLiu MSun CAo XYang AZhao HfalseRoot morphology, nitrogen metabolism and amino acid metabolism in soybean under low phosphorus stress.Phosphorus deficiency is a major influence on growth and development of soybean. Therefore, improving phosphorus utilization efficiency in soybean is a research priority for the soybean community. In this experiment, Liaodou 13 (high phosphorus utilization: HPE) and Tiefeng 3 (low phosphorus utilization: LPE) were used as test varieties. We investigated changes in root morphology, amino acid content, and content of key substances of the nitrogen metabolic pathway with normal phosphorus (0.5 mM) and low phosphorus (0.005 mM) treatments. The results showed that the root length, root surface area and number of lateral roots of HPE roots were higher than those of LPE roots under normal and low phosphorus conditions. The contents of different types of amino acids showed different trends in two 2024-01-01T00:00:00Z2024 Nov2025-04-18T17:08:06.493Z2025-04-07T04:41:52.224ZS-EPMC115771153956277710.1038/s41598-024-79876-0