{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["65(11)"],"submitter":["Li J"],"funding":["National Research Council"],"pubmed_abstract":["The recent advances in mass spectrometry (MS) technologies have enabled comprehensive lipid profiling in biological samples. However, the robustness and efficiency of MS-based lipidomics is compromised by the complexity of biological samples. High-field asymmetric waveform ion mobility spectrometry (FAIMS) is a technology that can continuously transmit one type of ion, independent of the mass-to-charge ratio. Here we present the development and application of LC-FAIMS-MS/MS-based platform for untargeted lipidomics. We used 3 optimally balanced compensation voltages, i.e., 29 V, 34 V and 39 V, to analyze all subclasses of glycerophospholipids. The reproducibility of the method was evaluated using reference standards. The reproducibility of retention times ranged from 0.9% to 1.5% RSD; where"],"journal":["Journal of lipid research"],"pagination":["100668"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11577210"],"repository":["biostudies-literature"],"pubmed_title":["Development of LC-FAIMS-MS and its application to lipidomics study of Acinetobacter baumannii infection."],"pmcid":["PMC11577210"],"pubmed_authors":["Haqqani AS","Li J","Stupak J","Williamson S","Chen W","Harris G","Zhou H","Chen R","Xu HH"],"additional_accession":[]},"is_claimable":false,"name":"Development of LC-FAIMS-MS and its application to lipidomics study of Acinetobacter baumannii infection.","description":"The recent advances in mass spectrometry (MS) technologies have enabled comprehensive lipid profiling in biological samples. However, the robustness and efficiency of MS-based lipidomics is compromised by the complexity of biological samples. High-field asymmetric waveform ion mobility spectrometry (FAIMS) is a technology that can continuously transmit one type of ion, independent of the mass-to-charge ratio. Here we present the development and application of LC-FAIMS-MS/MS-based platform for untargeted lipidomics. We used 3 optimally balanced compensation voltages, i.e., 29 V, 34 V and 39 V, to analyze all subclasses of glycerophospholipids. The reproducibility of the method was evaluated using reference standards. The reproducibility of retention times ranged from 0.9% to 1.5% RSD; where","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-06-02T09:20:21.652Z","creation":"2025-04-04T02:12:33.686Z"},"accession":"S-EPMC11577210","cross_references":{"pubmed":["39395788"],"doi":["10.1016/j.jlr.2024.100668"]}}