biostudies-literatureBartens MCBiotechnology and Biological Sciences Research Council136-149https://www.ebi.ac.uk/biostudies/studies/S-EPMC11577332biostudies-literatureUnknown30(6-8)The role of macrophage (MØ) cellular metabolism and reprogramming during TB infection is of great interest due to the influence of <i>Mycobacterium</i> spp. on MØ bioenergetics. Recent studies have shown that <i>M. tuberculosis</i> induces a TLR2-dependent shift towards aerobic glycolysis, comparable to the established LPS induced pro-inflammatory M1 MØ polarisation. Distinct differences in the metabolic profile of murine and human MØ indicates species-specific differences in bioenergetics. So far, studies examining the metabolic potential of bovine MØ are lacking, thus the basic bioenergetics of bovine and human MØ were explored in response to a variety of innate immune stimuli. Cellular energy metabolism kinetics were measured concurrently for both species on a Seahorse XFe96 platform toInnate immunityRespiratory bioenergetics is enhanced in human, but not bovine macrophages after exposure to <i>M. bovis</i> PPD: Exploratory insights into overall similar Cellular Metabolic Profiles.PMC11577332BB/P008461/1BB/N004590/1Gibson AJWerling DWillcocks SBartens MCfalseRespiratory bioenergetics is enhanced in human, but not bovine macrophages after exposure to <i>M. bovis</i> PPD: Exploratory insights into overall similar Cellular Metabolic Profiles.The role of macrophage (MØ) cellular metabolism and reprogramming during TB infection is of great interest due to the influence of <i>Mycobacterium</i> spp. on MØ bioenergetics. Recent studies have shown that <i>M. tuberculosis</i> induces a TLR2-dependent shift towards aerobic glycolysis, comparable to the established LPS induced pro-inflammatory M1 MØ polarisation. Distinct differences in the metabolic profile of murine and human MØ indicates species-specific differences in bioenergetics. So far, studies examining the metabolic potential of bovine MØ are lacking, thus the basic bioenergetics of bovine and human MØ were explored in response to a variety of innate immune stimuli. Cellular energy metabolism kinetics were measured concurrently for both species on a Seahorse XFe96 platform to2024-01-01T00:00:00Z2024 Aug2025-04-04T01:15:38.337Z2025-04-04T01:15:38.337ZS-EPMC115773323956350910.1177/17534259241296630