biostudies-literatureRao CArmy Medical University FoundationsMOST | National Natural Science Foundation of China (NSFC)National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC)MOST | National Natural Science Foundation of Chinae0045324https://www.ebi.ac.uk/biostudies/studies/S-EPMC11580396biostudies-literatureUnknown9(11)Melioidosis is a serious infectious disease caused by the Gram-negative bacterium <i>Burkholderia pseudomallei</i>. Recently, Rab32-dependent immune vesicles emerge as a critical defense pathway to restrict the intracellular <i>B. pseudomallei</i>. However, <i>B. pseudomallei</i> can evade host immune vesicles and survive in the cytoplasm, although this mechanism is not well understood. In this study, we found Rab32-dependent vesicles could effectively combat <i>B. pseudomallei</i> infection, but not all intracellular <i>B. pseudomallei</i> were encapsulated in Rab32-positive vesicles. To explore how <i>B. pseudomallei</i> counteracted the Rab32-dependent defense pathway, transcriptomic profiling of <i>B. pseudomallei</i> was performed to characterize the response dynamics during infectionmSphere<i>Burkholderia pseudomallei</i> BopE suppresses the Rab32-dependent defense pathway to promote its intracellular replication and virulence.PMC115803962021-2018-098,2020XQN068240264032270190,82272350Wen YLiao YYan JHuang JHu ZNan DWu PZheng SLi QWang LLiu WRao CZhang ZWang SYao CMao XQiao Jfalse<i>Burkholderia pseudomallei</i> BopE suppresses the Rab32-dependent defense pathway to promote its intracellular replication and virulence.Melioidosis is a serious infectious disease caused by the Gram-negative bacterium <i>Burkholderia pseudomallei</i>. Recently, Rab32-dependent immune vesicles emerge as a critical defense pathway to restrict the intracellular <i>B. pseudomallei</i>. However, <i>B. pseudomallei</i> can evade host immune vesicles and survive in the cytoplasm, although this mechanism is not well understood. In this study, we found Rab32-dependent vesicles could effectively combat <i>B. pseudomallei</i> infection, but not all intracellular <i>B. pseudomallei</i> were encapsulated in Rab32-positive vesicles. To explore how <i>B. pseudomallei</i> counteracted the Rab32-dependent defense pathway, transcriptomic profiling of <i>B. pseudomallei</i> was performed to characterize the response dynamics during infection2024-01-01T00:00:00Z2024 Nov2026-06-03T01:07:47.483Z2025-04-04T12:00:29.891ZS-EPMC115803963943183010.1128/msphere.00453-24