biostudies-literatureNishimori AMEXT | Japan Society for the Promotion of ScienceMEXT | Japan Society for the Promotion of Science (JSPS)e0021624https://www.ebi.ac.uk/biostudies/studies/S-EPMC11580432biostudies-literatureUnknown9(11)Oncogenic transformation of normal cells is caused by mutations and chromosomal abnormalities in cancer-related genes. Enzootic bovine leukosis (EBL) is a malignant B-cell lymphoma caused by bovine leukemia virus (BLV) infection in cattle. Although a small fraction of BLV-infected cattle develops EBL after a long latent period, the mechanisms for oncogenesis in EBL cattle remain largely unknown. In this study, we analyzed the types and patterns of somatic mutations in cancer cells from 36 EBL cases, targeting 21 cancer-related genes. Various somatic mutations were identified in eight genes, <i>TP53</i>, <i>KMT2D</i>, <i>CREBBP</i>, <i>KRAS</i>, <i>PTEN</i>, <i>NOTCH1</i>, <i>MYD88</i>, and <i>CARD11</i>. In addition, <i>TP53</i> gene was found to be mutated in 69.4% of EBL cases, with mostmSphereEffect of C-to-T transition at CpG sites on tumor suppressor genes in tumor development in cattle evaluated by somatic mutation analysis in enzootic bovine leukosis.PMC1158043219K1600823K14101Konnai SAndoh KNishimori AOkagawa TMatsuura YfalseEffect of C-to-T transition at CpG sites on tumor suppressor genes in tumor development in cattle evaluated by somatic mutation analysis in enzootic bovine leukosis.Oncogenic transformation of normal cells is caused by mutations and chromosomal abnormalities in cancer-related genes. Enzootic bovine leukosis (EBL) is a malignant B-cell lymphoma caused by bovine leukemia virus (BLV) infection in cattle. Although a small fraction of BLV-infected cattle develops EBL after a long latent period, the mechanisms for oncogenesis in EBL cattle remain largely unknown. In this study, we analyzed the types and patterns of somatic mutations in cancer cells from 36 EBL cases, targeting 21 cancer-related genes. Various somatic mutations were identified in eight genes, <i>TP53</i>, <i>KMT2D</i>, <i>CREBBP</i>, <i>KRAS</i>, <i>PTEN</i>, <i>NOTCH1</i>, <i>MYD88</i>, and <i>CARD11</i>. In addition, <i>TP53</i> gene was found to be mutated in 69.4% of EBL cases, with most2024-01-01T00:00:00Z2024 Nov2026-06-03T01:07:22.353Z2025-04-04T12:01:46.024ZS-EPMC115804323940426110.1128/msphere.00216-24