biostudies-literatureSchmidt MBundesministerium für Bildung und ForschungDeutsche ForschungsgemeinschaftUniversitätsklinikum RWTH AachenMedizinische Fakultät, RWTH Aachen UniversityElse Kröner-Fresenius-Stiftung164https://www.ebi.ac.uk/biostudies/studies/S-EPMC11580436biostudies-literatureUnknown16(1)<h4>Background</h4>Cancer-associated fibroblasts (CAFs) are essential components of the tumor microenvironment and play a critical role in cancer progression. Numerous studies have identified significant molecular differences between CAFs and normal tissue-associated fibroblasts (NAFs). In this study, we isolated CAFs and NAFs from liver tumors and conducted a comprehensive analysis of their DNA methylation profiles, integrating our finding with data from studies on other cancer types.<h4>Results</h4>Our analysis revealed that several CAF samples exhibited aberrant DNA methylation patterns, which corresponded with altered gene expression levels. Notably, DNA methylation at liver CAF-specific CpG sites was linked to survival outcomes in liver cancer datasets. An integrative analysis using pClinical epigeneticsCancer-associated fibroblasts reveal aberrant DNA methylation across different types of cancer.PMC11580436FibroMap CorsortiaForTra gGmbH PrickMeSFB 1506/1IZKF O3-3WA 1706/12-2WA1706/14-1GE 2811/4-1 within CRU344/417911533WA1706/11-1363055819/GRK2415Schmidt MCosta IGCramer TWagner WMaie TfalseCancer-associated fibroblasts reveal aberrant DNA methylation across different types of cancer.<h4>Background</h4>Cancer-associated fibroblasts (CAFs) are essential components of the tumor microenvironment and play a critical role in cancer progression. Numerous studies have identified significant molecular differences between CAFs and normal tissue-associated fibroblasts (NAFs). In this study, we isolated CAFs and NAFs from liver tumors and conducted a comprehensive analysis of their DNA methylation profiles, integrating our finding with data from studies on other cancer types.<h4>Results</h4>Our analysis revealed that several CAF samples exhibited aberrant DNA methylation patterns, which corresponded with altered gene expression levels. Notably, DNA methylation at liver CAF-specific CpG sites was linked to survival outcomes in liver cancer datasets. An integrative analysis using p2024-01-01T00:00:00Z2024 Nov2025-04-04T01:21:29.508Z2025-04-04T01:21:29.508ZS-EPMC115804363956796010.1186/s13148-024-01783-y