biostudies-literatureUnknownFrey ZDNIAID NIH HHSNINDS NIH HHSJamestown Canyon virus (JCV) is a bunyavirus and arbovirus responsible for neuroinvasive disease in the United States. Little is known about JCV pathogenesis, and no host factors required for cellular infection have been identified. Recently, we identified low-density lipoprotein receptor related protein 1 (Lrp1) as a host entry factor for two other bunyaviruses Rift Valley fever virus (RVFV) and Oropouche virus (OROV). Here, we assessed the role of Lrp1 in mediating JCV cellular infection of neurons. Both neuronal and non-neuronal immortalized cell lines deficient for Lrp1 displayed reduction in infection with JCV, and early stages of infection such as binding and internalization were impacted by lack of Lrp1. In primary rat neurons, Lrp1 was highly expressed, and the neurons were highly bioRxiv : the preprint server for biology2024.11.06.622176https://www.ebi.ac.uk/biostudies/studies/S-EPMC11580904biostudies-literatureLrp1 facilitates infection of neurons by Jamestown Canyon virus.PMC11580904R01 AI178378UC7 AI180311R01 AI161765R56 AI171920R01 AI169850R01 NS101100Sterling CEPrice DAFrey ZDCui XConnors KABrown GLeung DWRush REGanaie SAmarasinghe GKWills ZPHartman ALFatma FSchwarz MMfalseLrp1 facilitates infection of neurons by Jamestown Canyon virus.Jamestown Canyon virus (JCV) is a bunyavirus and arbovirus responsible for neuroinvasive disease in the United States. Little is known about JCV pathogenesis, and no host factors required for cellular infection have been identified. Recently, we identified low-density lipoprotein receptor related protein 1 (Lrp1) as a host entry factor for two other bunyaviruses Rift Valley fever virus (RVFV) and Oropouche virus (OROV). Here, we assessed the role of Lrp1 in mediating JCV cellular infection of neurons. Both neuronal and non-neuronal immortalized cell lines deficient for Lrp1 displayed reduction in infection with JCV, and early stages of infection such as binding and internalization were impacted by lack of Lrp1. In primary rat neurons, Lrp1 was highly expressed, and the neurons were highly 2024-01-01T00:00:00Z2024 Nov2026-06-03T03:13:21.951Z2025-04-05T09:30:22.387ZS-EPMC115809043957465110.1101/2024.11.06.622176