{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Zhebrun A"],"funding":["NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Nuclear RNAi in <i>C. elegans</i> induces a set of transgenerationally heritable marks of H3K9me3, H3K23me3, and H3K27me3 at the target genes. The function of H3K23me3 in the nuclear RNAi pathway is largely unknown due to the limited knowledge of H3K23 histone methyltransferase (HMT). In this study we identified SET-21 as a novel H3K23 HMT. By taking combined genetic, biochemical, imaging, and genomic approaches, we found that SET-21 functions synergistically with a previously reported H3K23 HMT SET-32 to deposit H3K23me3 at the native targets of germline nuclear RNAi. We identified a subset of native nuclear RNAi targets that are transcriptionally activated in the <i>set-21;set-32</i> double mutant. SET-21 and SET-32 are also required for robust transgenerational gene silencing induced by"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.11.05.622152"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11580914"],"repository":["biostudies-literature"],"pubmed_title":["Two H3K23 histone methyltransferases, SET-32 and SET-21, function synergistically to promote nuclear RNAi-mediated transgenerational epigenetic inheritance in <i>Caenorhabditis elegans</i>."],"pmcid":["PMC11580914"],"funding_grant_id":["S10 OD030286","R35 GM152219","R01 GM111752","P40 OD010440"],"pubmed_authors":["Corveleyn L","Sidoli S","Zhebrun A","Gu SG","Roy SG","Ni JZ"],"additional_accession":[]},"is_claimable":false,"name":"Two H3K23 histone methyltransferases, SET-32 and SET-21, function synergistically to promote nuclear RNAi-mediated transgenerational epigenetic inheritance in <i>Caenorhabditis elegans</i>.","description":"Nuclear RNAi in <i>C. elegans</i> induces a set of transgenerationally heritable marks of H3K9me3, H3K23me3, and H3K27me3 at the target genes. The function of H3K23me3 in the nuclear RNAi pathway is largely unknown due to the limited knowledge of H3K23 histone methyltransferase (HMT). In this study we identified SET-21 as a novel H3K23 HMT. By taking combined genetic, biochemical, imaging, and genomic approaches, we found that SET-21 functions synergistically with a previously reported H3K23 HMT SET-32 to deposit H3K23me3 at the native targets of germline nuclear RNAi. We identified a subset of native nuclear RNAi targets that are transcriptionally activated in the <i>set-21;set-32</i> double mutant. SET-21 and SET-32 are also required for robust transgenerational gene silencing induced by","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-05-23T03:24:56.361Z","creation":"2025-04-04T02:36:39.13Z"},"accession":"S-EPMC11580914","cross_references":{"pubmed":["39574755"],"doi":["10.1101/2024.11.05.622152"]}}