{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Moulin C"],"funding":["NEI NIH HHS"],"pubmed_abstract":["Optic nerve (ON) injury causes blindness in adult mammals as their retinal ganglion cells (RGCs) cannot regenerate axons. However, amphibian RGC axons do not experience the same regenerative failure. Studying the regeneration process of the ON in amphibians holds profound implications for regenerative medicine and human health. Using transgenic tadpoles and laser micro-optics, we developed a reproducible ON transection and regeneration model. Through microscopy, functional testing, TUNEL, EdU assays, and RNA-seq, we characterized the ON injury response and recovery. Our model suggests no chemoattractant gradient exists early in regeneration, with defasciculated axons sprouting in random directions from the globe-proximal cut end. Once individual axons reach the appropriate anatomical inser"],"journal":["Research square"],"pagination":["rs.3.rs-5085599"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11581122"],"repository":["biostudies-literature"],"pubmed_title":["Novel laser model of optic nerve transection provides valuable insights about the dynamics of optic nerve regeneration."],"pmcid":["PMC11581122"],"funding_grant_id":["P30 EY014801"],"pubmed_authors":["Bineshfar N","Groso D","Dvoriantchikova G","Swingle B","Pelaez D","Ivanov D","Moulin C","Zhang M","Martinez G"],"additional_accession":[]},"is_claimable":false,"name":"Novel laser model of optic nerve transection provides valuable insights about the dynamics of optic nerve regeneration.","description":"Optic nerve (ON) injury causes blindness in adult mammals as their retinal ganglion cells (RGCs) cannot regenerate axons. However, amphibian RGC axons do not experience the same regenerative failure. Studying the regeneration process of the ON in amphibians holds profound implications for regenerative medicine and human health. Using transgenic tadpoles and laser micro-optics, we developed a reproducible ON transection and regeneration model. Through microscopy, functional testing, TUNEL, EdU assays, and RNA-seq, we characterized the ON injury response and recovery. Our model suggests no chemoattractant gradient exists early in regeneration, with defasciculated axons sprouting in random directions from the globe-proximal cut end. Once individual axons reach the appropriate anatomical inser","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-04-08T19:39:27.711Z","creation":"2025-04-04T02:33:25.32Z"},"accession":"S-EPMC11581122","cross_references":{"pubmed":["39574891"],"doi":["10.21203/rs.3.rs-5085599/v1"]}}