{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(4)"],"submitter":["Panchal MH"],"funding":["Asthma, Allergy and Inflammation (AAIR) Charity","National Institute for Health Research (NIHR) Southampton Biomedical Research Centre","GSK plc"],"pubmed_abstract":["Lipids and their mediators have important regulatory functions in many cellular processes, including the innate antiviral response. The aim of this study was to compare the lipid membrane composition of in vitro differentiated primary bronchial epithelial cells (PBECs) with ex vivo bronchial brushings and to establish whether any changes in the lipid membrane composition affect antiviral defense of cells from donors without and with severe asthma. Using mass spectrometry, we showed that the lipid membrane of in vitro differentiated PBECs was deprived of polyunsaturated fatty acids (PUFAs) compared to ex vivo bronchial brushings. Supplementation of the culture medium with arachidonic acid (AA) increased the PUFA-content to more closely match the ex vivo membrane profile. Rhinovirus (RV16) i"],"journal":["Tissue barriers"],"pagination":["2300580"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11583602"],"repository":["biostudies-literature"],"pubmed_title":["Membrane lipid composition of bronchial epithelial cells influences antiviral responses during rhinovirus infection."],"pmcid":["PMC11583602"],"pubmed_authors":["Panchal MH","Nicholas BL","Goss VM","Childs CE","Thompson J","Djukanovic R","Rowan WC","Swindle EJ","Davies DE","Blume C","Pell TJ","Postle AD"],"additional_accession":[]},"is_claimable":false,"name":"Membrane lipid composition of bronchial epithelial cells influences antiviral responses during rhinovirus infection.","description":"Lipids and their mediators have important regulatory functions in many cellular processes, including the innate antiviral response. The aim of this study was to compare the lipid membrane composition of in vitro differentiated primary bronchial epithelial cells (PBECs) with ex vivo bronchial brushings and to establish whether any changes in the lipid membrane composition affect antiviral defense of cells from donors without and with severe asthma. Using mass spectrometry, we showed that the lipid membrane of in vitro differentiated PBECs was deprived of polyunsaturated fatty acids (PUFAs) compared to ex vivo bronchial brushings. Supplementation of the culture medium with arachidonic acid (AA) increased the PUFA-content to more closely match the ex vivo membrane profile. Rhinovirus (RV16) i","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Oct","modification":"2025-04-04T13:41:35.684Z","creation":"2025-04-04T13:41:35.684Z"},"accession":"S-EPMC11583602","cross_references":{"pubmed":["38179897"],"doi":["10.1080/21688370.2023.2300580"]}}