biostudies-literatureZhang YNatural Science Foundation of Shanghai Municipality2263https://www.ebi.ac.uk/biostudies/studies/S-EPMC11596105biostudies-literatureUnknown12(11)Rice is one of the most important staple crops worldwide. However, the bacterial blight of rice caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) poses a major threat to the production of rice. In this study, we isolated and identified the strain <i>Pseudomonas aeruginosa</i> SF416, which exhibited significant antagonistic activity against <i>Xoo</i>, from a soil sample collected in a winter wheat field in Shannanzhalang County, Tibet, China. The bacterial solution (BS) and cell-free supernatant (CFS) of SF416 had significant prevention effects for the bacterial blight of rice, with an efficacy of 45.1% and 34.18%, respectively, while they exhibited a slightly lower therapeutic efficiency of 31.64% and 25.09%. The genomic analysis showed that <i>P. aeruginosa</i> SF416 conMicroorganismsWhole Genome Sequencing and Comparative Genomic Analysis of <i>Pseudomonas aeruginosa</i> SF416, a Potential Broad-Spectrum Biocontrol Agent Against <i>Xanthomonas oryzae</i> pv. <i>oryzae</i>.PMC1159610522ZR1435500Tang ZZhu ZYu RZou LXiao YChen GQin TYan YLi XZhang YZhang CYin KXu ZfalseWhole Genome Sequencing and Comparative Genomic Analysis of <i>Pseudomonas aeruginosa</i> SF416, a Potential Broad-Spectrum Biocontrol Agent Against <i>Xanthomonas oryzae</i> pv. <i>oryzae</i>.Rice is one of the most important staple crops worldwide. However, the bacterial blight of rice caused by <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) poses a major threat to the production of rice. In this study, we isolated and identified the strain <i>Pseudomonas aeruginosa</i> SF416, which exhibited significant antagonistic activity against <i>Xoo</i>, from a soil sample collected in a winter wheat field in Shannanzhalang County, Tibet, China. The bacterial solution (BS) and cell-free supernatant (CFS) of SF416 had significant prevention effects for the bacterial blight of rice, with an efficacy of 45.1% and 34.18%, respectively, while they exhibited a slightly lower therapeutic efficiency of 31.64% and 25.09%. The genomic analysis showed that <i>P. aeruginosa</i> SF416 con2024-01-01T00:00:00Z2024 Nov2026-04-15T03:26:09.922Z2025-04-04T09:58:44.736ZS-EPMC115961053959765210.3390/microorganisms12112263