biostudies-literatureXu GChina Agriculture Research SystemChina Agriculture Research System of MOF and MARAGovernment Procurement ServicesCentral Public-interest Scientific Institution Basal Research Fund2255https://www.ebi.ac.uk/biostudies/studies/S-EPMC11596116biostudies-literatureUnknown12(11)<i>Phytophthora</i>-induced crop diseases, commonly known as "plant plagues", pose a significant threat to global food security. In this study, strain ASG80 was isolated from sisal roots and demonstrated a broad-spectrum antagonistic activity against several <i>Phytophthora</i> species and fungal pathogens. Strain ASG80 was identified as <i>Streptomyces luteireticuli</i> via phylogenetic analysis, digital DNA-DNA hybridization (dDDH), and average nucleotide identity (ANI). Whole-genome sequencing identified 40 biosynthetic gene clusters (BGCs) related to secondary metabolite production, including antimicrobial compounds. Strain ASG80 extract exhibited broad-spectrum inhibitory activity against <i>Phytophthora nicotianae</i>, <i>P. vignae</i>, <i>P. cinnamomi</i>, and <i>P. sojae</i>. Pot eMicroorganismsWhole Genome Sequencing and Biocontrol Potential of <i>Streptomyces luteireticuli</i> ASG80 Against <i>Phytophthora</i> Diseases.PMC11596116CARS-16212400821630042022005Yi KHe CZhu LLiang YLu YHuang XMa XTan SLiang MChen HWu WXu GfalseWhole Genome Sequencing and Biocontrol Potential of <i>Streptomyces luteireticuli</i> ASG80 Against <i>Phytophthora</i> Diseases.<i>Phytophthora</i>-induced crop diseases, commonly known as "plant plagues", pose a significant threat to global food security. In this study, strain ASG80 was isolated from sisal roots and demonstrated a broad-spectrum antagonistic activity against several <i>Phytophthora</i> species and fungal pathogens. Strain ASG80 was identified as <i>Streptomyces luteireticuli</i> via phylogenetic analysis, digital DNA-DNA hybridization (dDDH), and average nucleotide identity (ANI). Whole-genome sequencing identified 40 biosynthetic gene clusters (BGCs) related to secondary metabolite production, including antimicrobial compounds. Strain ASG80 extract exhibited broad-spectrum inhibitory activity against <i>Phytophthora nicotianae</i>, <i>P. vignae</i>, <i>P. cinnamomi</i>, and <i>P. sojae</i>. Pot e2024-01-01T00:00:00Z2024 Nov2026-04-15T03:22:27.049Z2025-04-04T09:58:52.645ZS-EPMC115961163959764410.3390/microorganisms12112255