{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang L"],"funding":["Ministry of Science, Innovation and Universities","Alberta Innovates Agri-Food Bioindustrial Innovation Program","MINECO","Alberta Innovates","CIBERFES","Maria de Maeztu Unit of Excellence grant","Canada Foundation for Innovation Major Sciences Initiatives Fund (CFI-MSI)","ICREA Academia Award","Genome Alberta","Canada Foundation for Innovation","Generalitat de Catalunya’s Agency AGAUR"],"pagination":["622"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11596266"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(11)"],"pubmed_abstract":["<b>Background/Objectives:</b> Targeted metabolomics is often criticized for the limited metabolite coverage that it offers. Indeed, most targeted assays developed or used by researchers measure fewer than 200 metabolites. In an effort to both expand the coverage and improve the accuracy of metabolite quantification in targeted metabolomics, we decided to develop a comprehensive liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay that could quantitatively measure more than 700 metabolites in serum or plasma. <b>Methods:</b> The developed assay makes use of chemical derivatization followed by reverse phase LC-MS/MS and/or direct flow injection MS (DFI-MS) in both positive and negative ionization modes to separate metabolites. Multiple reaction monitoring (MRM), in combination wit"],"journal":["Metabolites"],"pubmed_title":["A Comprehensive LC-MS Metabolomics Assay for Quantitative Analysis of Serum and Plasma."],"pmcid":["PMC11596266"],"funding_grant_id":["AB Innovat ABIP 222301549","MICIN/AEI/FEDER","42495","PID2020-114921RB-C21","222301549","CFI MSIF 42495","CEX2021-001234-M","CB16/ 10/00269","PRE2021-097813","2023-2027","GC MC5-TD","2021SGR00687","C MC5-TD"],"pubmed_authors":["Zheng J","Cruz M","Johnson M","Wishart DS","Andres-Lacueva C","Zhang L","Martinez-Huelamo M","Mandal R"],"additional_accession":[]},"is_claimable":false,"name":"A Comprehensive LC-MS Metabolomics Assay for Quantitative Analysis of Serum and Plasma.","description":"<b>Background/Objectives:</b> Targeted metabolomics is often criticized for the limited metabolite coverage that it offers. Indeed, most targeted assays developed or used by researchers measure fewer than 200 metabolites. In an effort to both expand the coverage and improve the accuracy of metabolite quantification in targeted metabolomics, we decided to develop a comprehensive liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay that could quantitatively measure more than 700 metabolites in serum or plasma. <b>Methods:</b> The developed assay makes use of chemical derivatization followed by reverse phase LC-MS/MS and/or direct flow injection MS (DFI-MS) in both positive and negative ionization modes to separate metabolites. Multiple reaction monitoring (MRM), in combination wit","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2026-04-08T19:37:00.401Z","creation":"2025-04-04T01:20:47.763Z"},"accession":"S-EPMC11596266","cross_references":{"pubmed":["39590858"],"doi":["10.3390/metabo14110622"]}}