{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Grunfeld J"],"funding":["Working Environment Research Fund (AMFF); and FFIKA, Focused Research Effort on Chemicals in the Working Environment from the Danish Government."],"pagination":["825"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11598809"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(11)"],"pubmed_abstract":["This work investigated the application of a reporter gene bioassay in assessing polycyclic aromatic hydrocarbon (PAH) exposure in trainee firefighters. In the PAH CALUX bioassay, the PAH-induced activation of the aryl hydrocarbon receptor in a reporter cell line is recorded by increased luminescence. A repeated measurement study was performed, collecting urine and skin wipe samples at two baseline sessions (spring and autumn) and after three firefighting sessions: one with wood fuel, one with gas fuel, and one without fire. The bioassay response was expressed as benzo[a]pyrene equivalents, which was compared to levels of 16 EPA criteria PAHs in skin wipe samples and 8 hydroxylated PAHs (OH-PAHs) in urine samples quantified by chromatography-tandem mass spectrometry techniques. Benzo[a]pyre"],"journal":["Toxics"],"pubmed_title":["Assessment of Polycyclic Aromatic Hydrocarbon Exposure in Trainee Firefighters Using PAH CALUX Bioassay."],"pmcid":["PMC11598809"],"funding_grant_id":["16-2022-03 (AMFF)"],"pubmed_authors":["Moller P","Vogel U","Kofoed-Sorensen V","Grunfeld J","Andersen MHG","Jensen SP"],"additional_accession":[]},"is_claimable":false,"name":"Assessment of Polycyclic Aromatic Hydrocarbon Exposure in Trainee Firefighters Using PAH CALUX Bioassay.","description":"This work investigated the application of a reporter gene bioassay in assessing polycyclic aromatic hydrocarbon (PAH) exposure in trainee firefighters. In the PAH CALUX bioassay, the PAH-induced activation of the aryl hydrocarbon receptor in a reporter cell line is recorded by increased luminescence. A repeated measurement study was performed, collecting urine and skin wipe samples at two baseline sessions (spring and autumn) and after three firefighting sessions: one with wood fuel, one with gas fuel, and one without fire. The bioassay response was expressed as benzo[a]pyrene equivalents, which was compared to levels of 16 EPA criteria PAHs in skin wipe samples and 8 hydroxylated PAHs (OH-PAHs) in urine samples quantified by chromatography-tandem mass spectrometry techniques. Benzo[a]pyre","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Nov","modification":"2025-04-19T04:21:17.178Z","creation":"2025-04-19T04:21:17.178Z"},"accession":"S-EPMC11598809","cross_references":{"pubmed":["39591003"],"doi":["10.3390/toxics12110825"]}}