{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15"],"submitter":["Mukhtar M"],"pubmed_abstract":["This study involves the development and molecular characterization of the isogenic markerless knockout mutant SG Δ<i>purB</i>, a genetically engineered live attenuated strain aimed at controlling <i>Salmonella</i> Gallinarum (SG) infection in poultry. The mutant was generated by deleting the <i>purB</i> gene using <i>λ</i>-Red recombination technology, impairing adenylosuccinate lyase, necessary for purine biosynthesis. An 1,180 bp deletion was engineered within the <i>purB</i> gene, leaving a residual 298 bp genomic scar resulting in a purine auxotrophic mutant. Phenotypically, SG Δ<i>purB</i> showed a 66.5% reduction in growth in LB broth compared to the wild-type strain and failed to grow in minimal media without adenosine. Growth was restored to near wild-type levels with 0.3 mM adenos"],"journal":["Frontiers in microbiology"],"pagination":["1467230"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11599157"],"repository":["biostudies-literature"],"pubmed_title":["Construction, molecular characterization, and safety assessment of <i>purB</i> mutant of <i>Salmonella</i> Gallinarum."],"pmcid":["PMC11599157"],"pubmed_authors":["Akhtar F","McClelland M","Ghafoor A","Rasheed MA","Mukhtar M"],"additional_accession":[]},"is_claimable":false,"name":"Construction, molecular characterization, and safety assessment of <i>purB</i> mutant of <i>Salmonella</i> Gallinarum.","description":"This study involves the development and molecular characterization of the isogenic markerless knockout mutant SG Δ<i>purB</i>, a genetically engineered live attenuated strain aimed at controlling <i>Salmonella</i> Gallinarum (SG) infection in poultry. The mutant was generated by deleting the <i>purB</i> gene using <i>λ</i>-Red recombination technology, impairing adenylosuccinate lyase, necessary for purine biosynthesis. An 1,180 bp deletion was engineered within the <i>purB</i> gene, leaving a residual 298 bp genomic scar resulting in a purine auxotrophic mutant. Phenotypically, SG Δ<i>purB</i> showed a 66.5% reduction in growth in LB broth compared to the wild-type strain and failed to grow in minimal media without adenosine. Growth was restored to near wild-type levels with 0.3 mM adenos","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024","modification":"2026-04-08T19:36:56.751Z","creation":"2025-04-19T04:25:58.494Z"},"accession":"S-EPMC11599157","cross_references":{"pubmed":["39606105"],"doi":["10.3389/fmicb.2024.1467230"]}}