biostudies-literatureUnknown15Mukhtar MThis study involves the development and molecular characterization of the isogenic markerless knockout mutant SG Δ<i>purB</i>, a genetically engineered live attenuated strain aimed at controlling <i>Salmonella</i> Gallinarum (SG) infection in poultry. The mutant was generated by deleting the <i>purB</i> gene using <i>λ</i>-Red recombination technology, impairing adenylosuccinate lyase, necessary for purine biosynthesis. An 1,180 bp deletion was engineered within the <i>purB</i> gene, leaving a residual 298 bp genomic scar resulting in a purine auxotrophic mutant. Phenotypically, SG Δ<i>purB</i> showed a 66.5% reduction in growth in LB broth compared to the wild-type strain and failed to grow in minimal media without adenosine. Growth was restored to near wild-type levels with 0.3 mM adenosFrontiers in microbiology1467230https://www.ebi.ac.uk/biostudies/studies/S-EPMC11599157biostudies-literatureConstruction, molecular characterization, and safety assessment of <i>purB</i> mutant of <i>Salmonella</i> Gallinarum.PMC11599157Akhtar FMcClelland MGhafoor ARasheed MAMukhtar MfalseConstruction, molecular characterization, and safety assessment of <i>purB</i> mutant of <i>Salmonella</i> Gallinarum.This study involves the development and molecular characterization of the isogenic markerless knockout mutant SG Δ<i>purB</i>, a genetically engineered live attenuated strain aimed at controlling <i>Salmonella</i> Gallinarum (SG) infection in poultry. The mutant was generated by deleting the <i>purB</i> gene using <i>λ</i>-Red recombination technology, impairing adenylosuccinate lyase, necessary for purine biosynthesis. An 1,180 bp deletion was engineered within the <i>purB</i> gene, leaving a residual 298 bp genomic scar resulting in a purine auxotrophic mutant. Phenotypically, SG Δ<i>purB</i> showed a 66.5% reduction in growth in LB broth compared to the wild-type strain and failed to grow in minimal media without adenosine. Growth was restored to near wild-type levels with 0.3 mM adenos2024-01-01T00:00:00Z20242026-04-08T19:36:56.751Z2025-04-19T04:25:58.494ZS-EPMC115991573960610510.3389/fmicb.2024.1467230