biostudies-literatureZajicek FUniversiteit AntwerpenCHDI FoundationResearch Foundation Flanders122-133https://www.ebi.ac.uk/biostudies/studies/S-EPMC11599348biostudies-literatureUnknown52(1)<h4>Purpose</h4>Positron emission tomography (PET) imaging of mutant huntingtin (mHTT) aggregates is a potential tool to monitor disease progression as well as the efficacy of candidate therapeutic interventions for Huntington's disease (HD). To date, the focus has been mainly on the investigation of ¹¹C radioligands; however, favourable ¹⁸F radiotracers will facilitate future clinical translation. This work aimed at characterising the novel [¹⁸F]CHDI-650 PET radiotracer using a combination of in vivo and in vitro approaches in a mouse model of HD.<h4>Methods</h4>After characterising [¹⁸F]CHDI-650 using in vitro autoradiography, we assessed in vivo plasma and brain radiotracer stability as well as kinetics through dynamic PET imaging in the heterEuropean journal of nuclear medicine and molecular imagingPreclinical evaluation of the novel [&lt;sup&gt;18&lt;/sup&gt;F]CHDI-650 PET ligand for non-invasive quantification of mutant huntingtin aggregates in Huntington's disease.PMC115993481229721NFFB210050A-11627Munoz-Sanjuan IStaelens SBertoglio DLiu LDe Lombaerde SVerhaeghe JVan Eetveldt ABard JDominguez CMiranda AZajicek FKhetarpal VfalsePreclinical evaluation of the novel [&lt;sup&gt;18&lt;/sup&gt;F]CHDI-650 PET ligand for non-invasive quantification of mutant huntingtin aggregates in Huntington's disease.<h4>Purpose</h4>Positron emission tomography (PET) imaging of mutant huntingtin (mHTT) aggregates is a potential tool to monitor disease progression as well as the efficacy of candidate therapeutic interventions for Huntington's disease (HD). To date, the focus has been mainly on the investigation of ¹¹C radioligands; however, favourable ¹⁸F radiotracers will facilitate future clinical translation. This work aimed at characterising the novel [¹⁸F]CHDI-650 PET radiotracer using a combination of in vivo and in vitro approaches in a mouse model of HD.<h4>Methods</h4>After characterising [¹⁸F]CHDI-650 using in vitro autoradiography, we assessed in vivo plasma and brain radiotracer stability as well as kinetics through dynamic PET imaging in the heter2024-01-01T00:00:00Z2024 Dec2025-04-22T14:24:11.324Z2025-04-06T01:00:19.722ZS-EPMC115993483919019710.1007/s00259-024-06880-x