biostudies-literatureUnknown2024Douarre CFast, accurate, and affordable bacterial identification methods are paramount for the timely treatment of infections, especially in resource-limited settings (RLS). However, today, only 1.3% of the sub-Saharan African diagnostic laboratories are performing clinical bacteriology. To improve this, diagnostic tools for RLS should prioritize simplicity, affordability, and ease of maintenance, as opposed to the costly equipment utilized for bacterial identification in high-income countries, such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In this work, we present a new high-throughput approach based on a simple wide-field (864 mm²) lensless imaging system allowing for the acquisition of a large portion of a Petri dish coupled with aInternational journal of biomedical imaging6465280https://www.ebi.ac.uk/biostudies/studies/S-EPMC11599477biostudies-literatureSimple Imaging System for Label-Free Identification of Bacterial Pathogens in Resource-Limited Settings.PMC11599477Marcoux PRVandenberg OFangazio MDavid DPicard EHadji EHardy LDouarre CBarbe BfalseSimple Imaging System for Label-Free Identification of Bacterial Pathogens in Resource-Limited Settings.Fast, accurate, and affordable bacterial identification methods are paramount for the timely treatment of infections, especially in resource-limited settings (RLS). However, today, only 1.3% of the sub-Saharan African diagnostic laboratories are performing clinical bacteriology. To improve this, diagnostic tools for RLS should prioritize simplicity, affordability, and ease of maintenance, as opposed to the costly equipment utilized for bacterial identification in high-income countries, such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). In this work, we present a new high-throughput approach based on a simple wide-field (864 mm²) lensless imaging system allowing for the acquisition of a large portion of a Petri dish coupled with a2024-01-01T00:00:00Z20242026-05-29T09:51:48.702Z2025-04-21T21:43:15.242ZS-EPMC115994773960627510.1155/2024/6465280