biostudies-literatureHodgens CNIGMS NIH HHSdiae015https://www.ebi.ac.uk/biostudies/studies/S-EPMC11599693biostudies-literatureUnknown6(2)Guard cell movements depend, in part, on the remodelling of vacuoles from a highly fragmented state to a fused morphology during stomata opening. Indeed, full opening of plant stomata requires vacuole fusion to occur. Fusion of vacuole membranes is a highly conserved process in eukaryotes, with key roles played by two multi-subunit complexes: HOPS (homotypic fusion and vacuolar protein sorting) and SNARE (soluble NSF attachment protein receptor). HOPS is a vacuole tethering factor that is thought to chaperone SNAREs from apposing vacuole membranes into a fusion-competent complex capable of rearranging membranes. In plants, recruitment of HOPS subunits to the tonoplast has been shown to require the presence of the phosphoinositide phosphatidylinositol 3-phosphate. However, chemically depletIn silico plantsModel-based inference of a dual role for HOPS in regulating guard cell vacuole fusion.PMC11599693T32 GM133366Khan IEnglish NJRojas-Pierce MPullen AMAkpa BSHodgens CFlaherty DTGillan LfalseModel-based inference of a dual role for HOPS in regulating guard cell vacuole fusion.Guard cell movements depend, in part, on the remodelling of vacuoles from a highly fragmented state to a fused morphology during stomata opening. Indeed, full opening of plant stomata requires vacuole fusion to occur. Fusion of vacuole membranes is a highly conserved process in eukaryotes, with key roles played by two multi-subunit complexes: HOPS (homotypic fusion and vacuolar protein sorting) and SNARE (soluble NSF attachment protein receptor). HOPS is a vacuole tethering factor that is thought to chaperone SNAREs from apposing vacuole membranes into a fusion-competent complex capable of rearranging membranes. In plants, recruitment of HOPS subunits to the tonoplast has been shown to require the presence of the phosphoinositide phosphatidylinositol 3-phosphate. However, chemically deplet2024-01-01T00:00:00Z20242026-05-29T09:51:47.764Z2025-04-21T21:43:10.058ZS-EPMC115996933961105310.1093/insilicoplants/diae015